GPRC5D Protein, Human, Recombinant (His & FLAG)

Orphan G-protein-coupled receptor family C group 5 member D (GPRC5D) has seven transmembrane segments forming the core of the receptor, as well as an intracellular C-tail and an extracellular N-terminal domain. GPRC5D is highly and selectively expressed on the surface of CD138+ multiple myeloma (MM) cells, whereas in normal tissues, a much lower expression is limited to plasma cells and certain hard keratinizing tissues. This makes GPRC5D a promising marker for monitoring tumor load and a target antigen for MM-specific T cell-engaging therapies such as bispecific antibodies and CAR-T cells.

规格	价格/CNY		货期	数量
20 μg	￥ 15,400	5日内发货
100 μg	￥ 54,000	5日内发货

规格

价格/CNY

货期

数量

20 μg

￥ 15,400

5日内发货

100 μg

￥ 54,000

5日内发货

生物活性

1. Immobilized Human GPRC5D Protein at 5 μg/mL (100 μL/well) can bind Anti-Human GPRC5D Antibody, Human IgG1, the EC50 is 20.6ng/mL（QC tested). 2.Loaded Anti-GPRC5D Antibody on Protein A Biosensor, can bind GPRC5D Protein with an affinity constant of 0.782 nM as determined in BLI assay (Sartorius Octet Red96e) (Routinely tested). 3.Captured Anti-GPRC5D antibody on anti-Human IgG Fc via CM5 Chip can bind GPRC5D protein with an affinity constant of 9.609nM as determined in a SPR assay (Routinely tested).

产品描述

种属	Human
表达系统	HEK293
标签	His,FLAG
蛋白编号	Q9NZD1
别名	GPRC5D
蛋白构建	A DNA sequence encoding the human GPRC5D (Q9NZD1) (Met1-Ser268) was expressed, with a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus.Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
蛋白纯度	≥ 75 % as determined by SDS-PAGE.
分子量	32.83 kDa (predicted)

内毒素	< 1.0 EU per μg protein as determined by the LAL method.
缓冲液	Supplied as solution in 10 mM HEPES, 50 mM NaCl, pH7.5 with glycerol as protectant. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
存储	Samples are stable for up to three months from date of receipt at -70℃. Store it under sterile conditions at -70℃ or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
运输方式	It is supplied and shipped as Solution with dry ice.
研究背景	Orphan G-protein-coupled receptor family C group 5 member D (GPRC5D) has seven transmembrane segments forming the core of the receptor, as well as an intracellular C-tail and an extracellular N-terminal domain. GPRC5D is highly and selectively expressed on the surface of CD138+ multiple myeloma (MM) cells, whereas in normal tissues, a much lower expression is limited to plasma cells and certain hard keratinizing tissues. This makes GPRC5D a promising marker for monitoring tumor load and a target antigen for MM-specific T cell-engaging therapies such as bispecific antibodies and CAR-T cells.