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Glycine oxidase Protein, Bacillus subtilis, Recombinant (His & SUMO)

Glycine oxidase Protein, Bacillus subtilis, Recombinant (His & SUMO)

产品编号 TMPH-00166
别名: thiO, Glycine oxidase

Glycine oxidase Protein, Bacillus subtilis, Recombinant (His & SUMO) is expressed in E. coli expression system with N-6xHis-SUMO tag. The predicted molecular weight is 56.9 kDa and the accession number is O31616.

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Glycine oxidase Protein, Bacillus subtilis, Recombinant (His & SUMO)
规格 价格/CNY 货期 数量
20 μg ¥ 2,290 20日内发货
100 μg ¥ 4,320 20日内发货
1 mg ¥ 14,600 20日内发货
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产品目录号及名称: Glycine oxidase Protein, Bacillus subtilis, Recombinant (His & SUMO) (TMPH-00166)
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产品描述 Glycine oxidase Protein, Bacillus subtilis, Recombinant (His & SUMO) is expressed in E. coli expression system with N-6xHis-SUMO tag. The predicted molecular weight is 56.9 kDa and the accession number is O31616.
种属 Bacillus subtilis
表达系统 E. coli
标签 N-6xHis-SUMO
蛋白编号 O31616
别名 thiO, Glycine oxidase
氨基酸序列 MKRHYEAVVIGGGIIGSAIAYYLAKENKNTALFESGTMGGRTTSAAAGMLGAHAECEERDAFFDFAMHSQRLYKGLGEELYALSGVDIRQHNGGMFKLAFSEEDVLQLRQMDDLDSVSWYSKEEVLEKEPYASGDIFGASFIQDDVHVEPYFVCKAYVKAAKMLGAEIFEHTPVLHVERDGEALFIKTPSGDVWANHVVVASGVWSGMFFKQLGLNNAFLPVKGECLSVWNDDIPLTKTLYHDHCYIVPRKSGRLVVGATMKPGDWSETPDLGGLESVMKKAKTMLPAIQNMKVDRFWAGLRPGTKDGKPYIGRHPEDSRILFAAGHFRNGILLAPATGALISDLIMNKEVNQDWLHAFRIDRKEAVQI
蛋白构建 1-369 aa
蛋白纯度 > 90% as determined by SDS-PAGE.
分子量 56.9 kDa (predicted)
缓冲液 Tris-based buffer, 50% glycerol
复溶方法 A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

运输方式

In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.

研究背景 Catalyzes the FAD-dependent oxidative deamination of various amines and D-amino acids to yield the corresponding alpha-keto acids, ammonia/amine, and hydrogen peroxide. Oxidizes sarcosine (N-methylglycine), N-ethylglycine and glycine. Can also oxidize the herbicide glyphosate (N-phosphonomethylglycine). Displays lower activities on D-alanine, D-valine, D-proline and D-methionine. Does not act on L-amino acids and other D-amino acids. Is essential for thiamine biosynthesis since the oxidation of glycine catalyzed by ThiO generates the glycine imine intermediate (dehydroglycine) required for the biosynthesis of the thiazole ring of thiamine pyrophosphate.

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Keywords

Glycine oxidase Protein, Bacillus subtilis, Recombinant (His & SUMO) thiO Glycine oxidase recombinant recombinant-proteins proteins protein

 

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