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GAPDH Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 37.9 kDa and the accession number is P16858.

| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 5 μg | ¥ 493 | 现货 | |
| 10 μg | ¥ 790 | 现货 | |
| 20 μg | ¥ 1,330 | 现货 | |
| 50 μg | ¥ 2,580 | 5日内发货 | |
| 100 μg | ¥ 5,170 | 5日内发货 |
| 生物活性 | Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
| 产品描述 | GAPDH Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 37.9 kDa and the accession number is P16858. |
| 种属 | Mouse |
| 表达系统 | E. coli |
| 标签 | N-His |
| 蛋白编号 | P16858 |
| 别名 | glyceraldehyde-3-phosphate dehydrogenase,Gapd |
| 蛋白构建 | A DNA sequence encoding the mouse GAPDH (P16858) (Met1-Glu333) was expressed with a polyhistidine tag at the N-terminus. Predicted N terminal: His |
| 蛋白纯度 | > 85 % as determined by SDS-PAGE |
| 分子量 | 37.9 kDa (predicted); 38 kDa (reducing condition, due to glycosylation) |
| 内毒素 | Please contact us for more information. |
| 蛋白性状 | Lyophilized powder |
| 缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 200 mM Arg, 10% gLycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| 复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. |
| 研究背景 | Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions. |