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GAPDH Protein, Human, Recombinant (His)

产品编号 TMPY-02446

GAPDH Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 38 kDa and the accession number is P04406.

GAPDH Protein, Human, Recombinant (His)

GAPDH Protein, Human, Recombinant (His)

产品编号 TMPY-02446
GAPDH Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 38 kDa and the accession number is P04406.
规格价格库存数量
100 μg
¥ 3,820
5日内发货
1 mg
¥ 9,870
5日内发货
大包装 & 定制
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实验操作小课堂
常见问题解答
抑制剂加 DMSO 配成母液之后,能否放在常温的环境保存1周?
不建议这么做,母液配置好分装保存,建议存于 -20℃ 或 -80℃,尽量避免反复冻融。如果您订购的规格很大,可以考虑分装粉末保存,固体粉末是比较稳定的,-20℃ 可以保存三年。
超声应使用什么仪器?
建议使用“超声清洗仪”,不推荐用“超声破碎仪”。
母液配置的计算方法?
(1) c=n/v=m/M/v 浓度=物质的量/体积=质量/相对分子质量/体积 (2) 官网产品详情页下方有溶液配制表格,可以参考计算好的加入溶剂体积
加药后检测结果没有作用,是不是产品质量有问题?
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产品信息

生物活性
Measured by its ability to catalyze D-glyceraldehyde 3-phosphate to 3-phosphoglycerate , the specific activity is >2500pmols/min/ug.
产品描述
GAPDH Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 38 kDa and the accession number is P04406.
种属
Human
表达系统
E. coli
标签N-His
蛋白编号P04406
别名
HEL-S-162eP,glyceraldehyde-3-phosphate dehydrogenase,GAPD,G3PD
蛋白构建
A DNA sequence encoding the human GAPDH (P04406) (Met 1-Glu 335) was expressed, with a polyhistidine tag at the N-terminus. Predicted N terminal: Met
蛋白纯度
> 93 % as determined by SDS-PAGE
分子量38 kDa (predicted); 38 kDa (reducing conditions)
内毒素Please contact us for more information.
缓冲液Supplied as sterile 50 mM Tris, 30% glycerol, pH 7.5.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.

SCI 文献

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