Name: EDF1 Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPJ-00693
Rating: 4.5 (1 reviews)

规格

价格

库存

数量

5 μg

¥ 693

6-8日内发货

10 μg

¥ 1,170

6-8日内发货

20 μg

¥ 1,860

5日内发货

50 μg

¥ 3,470

5日内发货

100 μg

¥ 4,930

5日内发货

200 μg

¥ 7,350

5日内发货

500 μg

¥ 12,100

5日内发货

1 mg

¥ 17,300

5日内发货

生物活性

产品描述	Endothelial Differentiation-Related Factor 1 (EDF1) is a 148 amino acid transcriptional coactivator that contains 1 HTH cro/C1-type DNA-binding domain. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. When endothelial cells are induced to differentiate in vitro, EDF1 is downregulated, leading to inhibition of cell growth and cell polarization. EDF1 binds calmodulin thorough its IQ domain and regulates nitric oxide synthase activity through calmodulin sequestration in the cytoplasm. Though ubiquitously expressed, EDF1 is most abundant in adult liver, heart, adipose tissues, intestine and pancreas. In fetal tissues, EDF1 is most abundant in kidney. There are two isoforms of EDF1 that are produced as a result of alternative splicing events.
生物活性	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
研究背景	Endothelial Differentiation-Related Factor 1 (EDF1) is a 148 amino acid transcriptional coactivator that contains 1 HTH cro/C1-type DNA-binding domain. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. When endothelial cells are induced to differentiate in vitro, EDF1 is downregulated, leading to inhibition of cell growth and cell polarization. EDF1 binds calmodulin thorough its IQ domain and regulates nitric oxide synthase activity through calmodulin sequestration in the cytoplasm. Though ubiquitously expressed, EDF1 is most abundant in adult liver, heart, adipose tissues, intestine and pancreas. In fetal tissues, EDF1 is most abundant in kidney. There are two isoforms of EDF1 that are produced as a result of alternative splicing events.
种属	Human
表达系统	E. coli
标签	C-6xHis
蛋白编号	O60869
氨基酸序列	Ala2-Lys148
蛋白构建	Ala2-Lys148
蛋白纯度	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
别名	Multiprotein-Bridging Factor 1, MBF1, Endothelial Differentiation-Related Factor 1, EDF-1, EDF1
内毒素	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.

化学信息

分子量	16-20 KDa (reducing condition)

储存&溶解度

运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
存储	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

EDF1 Protein, Human, Recombinant (His)

EDF1 Protein, Human, Recombinant (His)

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