CD16b Protein, Human, Recombinant (NA2, HEK293, His)

产品编号 TMPY-00701

CD16b Protein, Human, Recombinant (NA2, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.2 kDa and the accession number is O75015.2.

CD16b Protein, Human, Recombinant (NA2, HEK293, His)

产品编号 TMPY-00701

CD16b Protein, Human, Recombinant (NA2, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.2 kDa and the accession number is O75015.2.

规格	价格	库存
100 μg	¥ 3,820	现货
200 μg	¥ 6,750	5日内发货
500 μg	¥ 14,280	5日内发货

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产品信息

生物活性	1.Loaded Human FcγRIIIB / CD16b (NA2) recombinant protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 8.3 μM as determined in a BLI assay (Sartorius Octet Red384) (QC tested). 2.Captured Human FcγRIIIB / CD16b (NA2) recombinant protein on Anti-His Chip can bind IgG1 with an affinity constant of 2.6 μM as determined in an SPR assay (Biacore T200) (Routinely tested). 3.Captured IgG1 on proteinA Chip can bind Human FcγRIIIB/CD16b (NA2) recombinant protein with an affinity constant of 0.22 μM as determined in an SPR assay (Biacore T200) (Routinely tested). 4.The purity of Human CD16b/FCGR3B Protein (His Tag, NA2 allotype) is more than 95% and the molecular weight of this protein is around 33-43 kDa verified by SEC-MALS.
产品描述	CD16b Protein, Human, Recombinant (NA2, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.2 kDa and the accession number is O75015.2.
种属	Human
表达系统	HEK293 Cells
标签	C-His
蛋白编号	O75015-2
别名	FCRIIIb,FCRIII,FCR-10,FCGR3,FCG3,Fc γ RIIIb,Fc gamma RIIIb,Fc fragment of IgG receptor IIIb,CD16b,CD16
蛋白构建	A DNA sequence encoding the human CD16b (O75015.2) (Met 1-Ser 200) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Gly 17
蛋白纯度	> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
分子量	22.2 kDa (predicted); 46 kDa (reducing condition, due to glycosylation)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.