Name: ATF1 Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPJ-00886
Rating: 4.5 (1 reviews)

规格

价格

库存

数量

5 μg

¥ 693

6-8日内发货

10 μg

¥ 1,170

6-8日内发货

20 μg

¥ 1,860

5日内发货

50 μg

¥ 3,470

5日内发货

100 μg

¥ 4,930

5日内发货

200 μg

¥ 7,350

5日内发货

500 μg

¥ 12,100

5日内发货

1 mg

¥ 18,900

5日内发货

生物活性

产品描述	Cyclic AMP-dependent transcription factor ATF-1(ATF1) which contains 1 bZIP (basic-leucine zipper) domain and 1 KID (kinase-inducible) domain, belongs to the bZIP family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. ATF1 binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It also binds to the Tax-responsive element (TRE) of HTLV-I. ATF1 mediates PKA-induced stimulation of CRE-reporter genes, represses the expression of FTH1 and other antioxidant detoxification genes, triggers cell proliferation and transformation. ATF1 is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and CDK3. Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation.
生物活性	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
研究背景	Cyclic AMP-dependent transcription factor ATF-1(ATF1) which contains 1 bZIP (basic-leucine zipper) domain and 1 KID (kinase-inducible) domain, belongs to the bZIP family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. ATF1 binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It also binds to the Tax-responsive element (TRE) of HTLV-I. ATF1 mediates PKA-induced stimulation of CRE-reporter genes, represses the expression of FTH1 and other antioxidant detoxification genes, triggers cell proliferation and transformation. ATF1 is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and CDK3. Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation.
种属	Human
表达系统	E. coli
标签	C-6xHis
蛋白编号	P18846
氨基酸序列	Met1-Val271
蛋白构建	Met1-Val271
蛋白纯度	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
别名	Cyclic AMP-dependent transcription factor ATF-1, cAMP-dependent transcription factor ATF-1, Activating transcription factor 1
内毒素	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.

化学信息

分子量	32 KDa (reducing condition)

储存&溶解度

运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
存储	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

ATF1 Protein, Human, Recombinant (His)

ATF1 Protein, Human, Recombinant (His)

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