规格

价格

库存

数量

100 U

¥ 672

现货

生物活性

产品描述	Enterokinase is a member of the trypsin family of serine proteases. The precursor protein is cleaved into two chains which then forms a heterodimer linked by a disulfide bond. The heavy chain anchors enterokinase in the intestinal brush border membrane and the light chain is the catalytic subunit, which initiates conversion activation of a subset of pancreatic proteolytic proenzymes. Enterokinase is the physiological activator of trypsinogen and has a specificity for the sequence (Asp)4-Lys-Ile. The mature trypsin in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases. In addition, Enterokinase is a tool protease widely utilized in the cleavage of recombinant fusion proteins.
生物活性	One unit is defined as the amount of enzyme needed to cleave 50μg fusion protein in 16 hours at 20℃ in a buffer containing 20 mM Tris-HCl, 50 mM NaCl, and 2 mM CaCl2>, pH 7.4.
研究背景	Enterokinase is a member of the trypsin family of serine proteases. The precursor protein is cleaved into two chains which then forms a heterodimer linked by a disulfide bond. The heavy chain anchors enterokinase in the intestinal brush border membrane and the light chain is the catalytic subunit, which initiates conversion activation of a subset of pancreatic proteolytic proenzymes. Enterokinase is the physiological activator of trypsinogen and has a specificity for the sequence (Asp)4-Lys-Ile. The mature trypsin in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases. In addition, Enterokinase is a tool protease widely utilized in the cleavage of recombinant fusion proteins.
种属	Bovine
表达系统	P. pastoris (Yeast)
标签	Tag Free
蛋白构建	A DNA sequence encoding the light chain (Ile 801-His 1035) of bovine Enterokinase (NP_776864) was expressed.
蛋白纯度	> 95%, as determined by SDS-PAGE and SEC-HPLC Analysis
蛋白性状	Lyophilized powder
复溶方法	Resuspend the enzyme powder with sterile water. Keep reconstituted enzyme at -20℃ in aliquots.
别名	EK, Enterokinase, PRSS7, Enteropeptidase, EC 3.4.21.9, Serine protease 7, ENTK, MGC133046.
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	26.1kDa (predicted); 45kDa (reducing condition, due to glycosylation)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

Enterokinase/EK Protein, Bovine, Recombinant

Enterokinase/EK Protein, Bovine, Recombinant

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