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Rabbit Anti-Mouse IgG3 Secondary Antibody (7Z583)
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产品编号 TMAY-00008
别名 IGHG3
Rabbit Anti-Mouse IgG3 Secondary Antibody (7Z583) 是一种 Rabbit 抗体，靶向 IgG3。Rabbit Anti-Mouse IgG3 Secondary Antibody (7Z583) 可用于 ELISA。
Rabbit Anti-Mouse IgG3 Secondary Antibody (7Z583)
331 Rabbit Anti-Mouse IgG3 Secondary Antibody (7Z583)
还可以产品编号 TMAY-00008 别名 IGHG3
Rabbit Anti-Mouse IgG3 Secondary Antibody (7Z583) 是一种 Rabbit 抗体，靶向 IgG3。Rabbit Anti-Mouse IgG3 Secondary Antibody (7Z583) 可用于 ELISA。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 20 μL | ¥ 580 | 5日内发货 | |
| 100 μL | ¥ 1,980 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Rabbit Anti-Mouse IgG3 Secondary Antibody (7Z583) is a Rabbit antibody targeting IgG3. Rabbit Anti-Mouse IgG3 Secondary Antibody (7Z583) can be used in ELISA. |
| 别名 | IGHG3 |
| Ig Type | Rabbit IgG |
| 克隆号 | 7Z583 |
| 交叉反应 | Mouse |
| 验证活性 | 1. ELISA of mouse immunoglobulins shows Rabbit Anti-Mouse IgG3 Secondary Antibody reacts only to mouse IgG3. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM. The plate was coated with 0.1 μg/mL of different mouse immunoglobulins. 0.1 μg/mL of Rabbit Anti-Mouse IgG3 Secondary Antibody was used as the primary antibody. An HRP conjugated anti-Rabbit IgG as the secondary antibody. 2. Immunohistochemical analysis of PFA-fixed paraffin-embedded human liver tissue sections, with VEGF primary antibody (1/500) for 1 hours at 37°C. An HRP-conjugated Rabbit Anti-Mouse IgG3 Secondary Antibody (1/6400) was used as the secondary antibody. (Validation Experiment) 3. Immunohistochemical analysis of PFA-fixed paraffin-embedded human prostate tissue sections, with KRT7 primary antibody (1/200) for 1 hours at 37°C. An HRP-conjugated Rabbit Anti-Mouse IgG3 Secondary Antibody (1/3200) was used as the secondary antibody. (Validation Experiment) 4. Anti-ENO3 mouse monoclonal antibody at 1:1000 dilution. -Lane A: HepG2 Whole Cell Lysate. -Lane B: HeLa Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Rabbit Anti-Mouse IgG3/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:47 kDa. -Observed band size:47 kDa(Validation Experiment) |
| 应用 | ELISA |
| 推荐剂量 | ELISA: 1:5000-1:10000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse IgG3. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | IGHG3 (Immunoglobulin Heavy Constant Gamma 3 (G3m Marker), also known as IgG3) is a Protein Coding gene. Ig gamma-3 chain C region is a protein that in humans is encoded by the IGHG3 gene. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Murine immunoglobulin G (IgG) plays an important role in mediating protective immune responses to malaria. Diseases associated with IGHG3 include Heavy Chain Disease and Gamma Heavy Chain Disease. Among its related pathways are IL4-mediated signaling events and the Creation of C4 and C2 activators. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | HRP |
| 可提供抗体种类 | 2 |
| 免疫原 | Recombinant Protein: Mouse IgG3 Protein |
| 抗原种属 | Mouse |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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