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Rabbit Anti-Mouse IgG2b Secondary Antibody-HRP (9P156) 是一种 HRP-conjugated Rabbit 抗体,靶向 IgG2b。Rabbit Anti-Mouse IgG2b Secondary Antibody-HRP (9P156) 可用于 WB,ELISA。
Rabbit Anti-Mouse IgG2b Secondary Antibody-HRP (9P156) 是一种 HRP-conjugated Rabbit 抗体,靶向 IgG2b。Rabbit Anti-Mouse IgG2b Secondary Antibody-HRP (9P156) 可用于 WB,ELISA。
产品描述 | Rabbit Anti-Mouse IgG2b Secondary Antibody-HRP (9P156) is a HRP-conjugated Rabbit antibody targeting IgG2b. Rabbit Anti-Mouse IgG2b Secondary Antibody-HRP (9P156) can be used in WB,ELISA. |
Ig Type | Monoclonal Rabbit IgG |
克隆号 | 9P156 |
交叉反应 | Mouse |
验证活性 | 1. ELISA of mouse immunoglobulins shows Rabbit Anti-Mouse IgG2b Secondary Antibody (HRP), Rabbit MAb reacts only to mouse IgG2b. No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM, Rabbit IgG, Human IgG. The plate was coated with 0.1 μg/mL of different mouse immunoglobulins. 0.5 μg/mL of Rabbit Anti-Mouse IgG2b Secondary Antibody (HRP), Rabbit MAb was used as secondary antibody. 2. Anti-IL-3R alpha/CD123 monoclonal antibody at 1:1000 dilution. -Lane A: Recombinant Human IL-3R alpha/CD123 Protein 100ng -Secondary -Rabbit Anti-Mouse IgG2b/HRP at 1/1000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. (Validation Experiment) |
应用 | WB,ELISA |
推荐剂量 | WB: 0.02-1 μg/mL; ELISA: 0.1-1 μg/mL |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse IgG2b (rM IgG2b; 51095-MNAHP01867-2Glu97-Lys335) and conjugated with horseradish-peroxidase (HRP). |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
偶联 | HRP |
其他可选修饰符 | Unconjugated |
可提供抗体种类 | 2 |
免疫原 | Recombinant Mouse IgG2b-Fc Protein (TMPY-02695) |
抗原种属 | Mouse |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. Keep away from direct sunlight. |
运输方式 | Shipping with blue ice. |
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