购物车
- 全部删除
您的购物车当前为空
Rabbit Anti-Mouse IgG1 Secondary Antibody-HRP (7L454)
还可以产品编号 TMAY-00001H
Rabbit Anti-Mouse IgG1 Secondary Antibody-HRP (7L454) 是一种 HRP-conjugated Rabbit 抗体，靶向 IgG1。Rabbit Anti-Mouse IgG1 Secondary Antibody-HRP (7L454) 可用于 WB,ELISA,IHC-P。
Rabbit Anti-Mouse IgG1 Secondary Antibody-HRP (7L454)
Rabbit Anti-Mouse IgG1 Secondary Antibody-HRP (7L454)
还可以产品编号 TMAY-00001H
Rabbit Anti-Mouse IgG1 Secondary Antibody-HRP (7L454) 是一种 HRP-conjugated Rabbit 抗体，靶向 IgG1。Rabbit Anti-Mouse IgG1 Secondary Antibody-HRP (7L454) 可用于 WB,ELISA,IHC-P。
产品信息正在更新中，如果您想购买，请点击大包装&定制按钮.
大包装 & 定制
联系我们获取更多批次信息
资源下载
产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Rabbit Anti-Mouse IgG1 Secondary Antibody-HRP (7L454) is a HRP-conjugated Rabbit antibody targeting IgG1. Rabbit Anti-Mouse IgG1 Secondary Antibody-HRP (7L454) can be used in WB,ELISA,IHC-P. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 7L454 |
| 交叉反应 | Mouse |
| 验证活性 | 1. ELISA of mouse immunoglobulins shows Rabbit Anti-Mouse IgG1 Secondary Antibody (HRP), Rabbit MAb reacts only to mouse IgG1. No cross reactivity with mouse IgG2a, IgG2b, IgG3, IgM, Rabbit IgG, Human IgG. The plate was coated with 0.1 μg/mL of different mouse immunoglobulins. 0.5 μg/mL of Rabbit Anti-Mouse IgG1 Secondary Antibody (HRP), Rabbit MAb was used as the secondary antibody. 2. Immunohistochemical analysis of PFA-fixed paraffin-embedded human stomach carcinoma tissue sections, with Claudin18.1 primary antibody (1:200) for 1 hours at 37°C. An HRP-conjugated Rabbit Anti-Mouse IgG1 Secondary Antibody (1:1600) was used as the secondary antibody. 3. Immunohistochemical analysis of PFA-fixed paraffin-embedded human prostate tissue sections, with KLK15 primary antibody (1:60) for 1 hours at 37°C. An HRP-conjugated Rabbit Anti-Mouse IgG1 Secondary Antibody (1:1600) was used as the secondary antibody. 4. Anti-Carboxypeptidase A monoclonal antibody at 1:1000 dilution. -Lane A: Recombinant Human Carboxypeptidase A Protein 100ng -Secondary -Rabbit Anti-Mouse IgG1/HRP at 1/1000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. |
| 应用 | WB,ELISA,IHC-P |
| 推荐剂量 | WB: 0.1-1μg/mL; ELISA: 0.1-1 μg/mL; IHC-P: 0.3-2.5 μg/mL |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse IgG1 (rM IgG1; 10690-MNAHP01868-1Val98-Lys324,C102S), and conjugated with horseradish-peroxidase (HRP). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (5 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues. Protein G or Protein A on the surface of certain Staphylococcal and Streptococcal strains specifically binds with the Fc region of IgGs, and has numerous applications in biotechnology as a reagent for affinity purification. Recombinant IgG Fc Region is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases. |
| 偶联 | HRP |
| 其他可选修饰符 | Unconjugated |
| 可提供抗体种类 | 2 |
| 免疫原 | Recombinant Mouse IgG1-Fc Protein |
| 抗原种属 | Mouse |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. Keep away from direct sunlight. |
| 运输方式 | Shipping with blue ice. |
SCI 文献
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
评论列表
预览
Related Tags: buy Rabbit Anti-Mouse IgG1 Secondary Antibody-HRP (7L454) | purchase Rabbit Anti-Mouse IgG1 Secondary Antibody-HRP (7L454) | Rabbit Anti-Mouse IgG1 Secondary Antibody-HRP (7L454) cost | order Rabbit Anti-Mouse IgG1 Secondary Antibody-HRP (7L454)
嗨！有任何问题？点我咨询
评论内容