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Histone H3 Nuclear Loading Control Antibody (6Z247)
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产品编号 TMAB-00865
Histone H3 Nuclear Loading Control Antibody (6Z247) 是一种 Mouse 抗体，靶向 Histone H3 Nuclear Loading Control。Histone H3 Nuclear Loading Control Antibody (6Z247) 可用于 WB,IHC-P,IHC-Fr,IF。
Histone H3 Nuclear Loading Control Antibody (6Z247)
Histone H3 Nuclear Loading Control Antibody (6Z247)
还可以产品编号 TMAB-00865
Histone H3 Nuclear Loading Control Antibody (6Z247) 是一种 Mouse 抗体，靶向 Histone H3 Nuclear Loading Control。Histone H3 Nuclear Loading Control Antibody (6Z247) 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 670 | 5日内发货 | |
| 100 μL | ¥ 970 | 5日内发货 | |
| 500 μL | ¥ 3,985 | 5日内发货 |
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产品介绍
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抗原信息
| 产品描述 | Histone H3 Nuclear Loading Control Antibody (6Z247) is a Mouse antibody targeting Histone H3 Nuclear Loading Control. Histone H3 Nuclear Loading Control Antibody (6Z247) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 克隆号 | 6Z247 |
| 交叉反应 | Human,Mouse,Rat,Hamster (predicted:Bee) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-00865) at 1:500 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody (YF488) for 90 minutes, and DAPI for nucleus staining. 2. Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-00865) at 1:300 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody (YF488) for 90 minutes, and DAPI for nucleus staining. 3. Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Histone H3/HIST3H3) Monoclonal Antibody, Unconjugated (TMAB-00865) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 90 minutes, and DAPI for nucleus staining. 4. Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-00865) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-00865) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (TMAB-00865) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Purple, ), DAPI (blue) was used to stain the cell nucleus. 7. Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (TMAB-00865) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Purple, ), DAPI (blue) was used to stain the cell nucleus. 8. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (TMAB-00865) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Purple, ), DAPI (blue) was used to stain the cell nucleus. 9. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (TMAB-00865) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Purple, ), DAPI (blue) was used to stain the cell nucleus. 10. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (TMAB-00865) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Purple, ), DAPI (blue) was used to stain the cell nucleus. 11. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (TMAB-00865) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Purple, ), DAPI (blue) was used to stain the cell nucleus. 12. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (TMAB-00865) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Purple, ), DAPI (blue) was used to stain the cell nucleus. 13. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (TMAB-00865) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Purple, ), DAPI (blue) was used to stain the cell nucleus. 14. 25 μg total protein per Lane of various lysates probed with Histone H3 monoclonal antibody, unconjugated (TMAB-00865) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 15. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of TMAB-00865) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 16. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of TMAB-00865) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 17. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of TMAB-00865) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 18. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of TMAB-00865) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 19. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of TMAB-00865) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 20. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum； Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of TMAB-00865) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 21. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum； Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of TMAB-00865) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 22. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum； Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of TMAB-00865) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 23. Paraformaldehyde-fixed, paraffin embedded Rat Liver； Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of TMAB-00865) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 24. Paraformaldehyde-fixed, paraffin embedded Human Liver； Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of TMAB-00865) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 25. Paraformaldehyde-fixed, paraffin embedded Rat Kidney； Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of TMAB-00865) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 26. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer； Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of TMAB-00865) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 27. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer； Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of TMAB-00865) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 28. Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer； Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of TMAB-00865) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 29. Paraformaldehyde-fixed, paraffin embedded Human Pancreas； Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (ascites of TMAB-00865) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 30. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (TMAB-00865) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. 31. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (TMAB-00865) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. |
| 应用 | |
| 推荐剂量 | WB: 1:2000-20000; IHC-P: 1:1000-2000; IHC-Fr: 1:1000-2000; IF: 1:100-500 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Nucleus. Chromosome. Note=Localizes to both the large, transcriptionally active, somatic macronucleus (MAC) and the small, transcriptionally inert, germ line micronucleus (MIC). |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein G purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. |
| 免疫原 | KLH conjugated synthetic peptide: human Histone H3 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | ChIP antibodies,Methylated |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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