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Goat Anti-Rabbit IgG H&L Secondary Antibody-IRDye 800cw 是一种 IRDye 800cw-conjugated Goat 抗体,靶向 IgG H&L。Goat Anti-Rabbit IgG H&L Secondary Antibody-IRDye 800cw 可用于 WB。
Goat Anti-Rabbit IgG H&L Secondary Antibody-IRDye 800cw 是一种 IRDye 800cw-conjugated Goat 抗体,靶向 IgG H&L。Goat Anti-Rabbit IgG H&L Secondary Antibody-IRDye 800cw 可用于 WB。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
100 μL | ¥ 875 | 5日内发货 |
产品描述 | Goat Anti-Rabbit IgG H&L Secondary Antibody-IRDye 800cw is a IRDye 800cw-conjugated Goat antibody targeting IgG H&L. Goat Anti-Rabbit IgG H&L Secondary Antibody-IRDye 800cw can be used in WB. |
别名 | IRDye® 800CW Goat anti-Rabbit IgG H&L, Immunoglobulin G |
Ig Type | IgG1 |
交叉反应 | Rabbit |
验证活性 | 1. Sample: Lane 1,4,7: Mouse Testis tissue lysates Lane 2,5,8: Rat Testis tissue lysates Lane 3,6,9: Human MCF-7 cell lysates Primary: Anti-beta-Actin at 1/1:2000 dilution Secondary: IRDye800CW Goat anti-Rabbit IgG at 1:50000~200000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa ![]() |
应用 | |
推荐剂量 | WB: 1:10000-30000 |
抗体种类 | Polyclonal |
宿主来源 | Goat |
构建方式 | Polyclonal Antibody |
纯化方式 | Protein G purified |
性状 | Liquid |
缓冲液 | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
浓度 | 1.0 mg/ml |
研究背景 | IRDye800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L), Highly Cross Adsorbed Producer: Li-Cor(USA) Product Number: 926-32211 Fluorophore: IRDye 800CW(MW: 1166) Excitation Wavelength: 778nm(in PBS) Emission Wacelegth: 795nm(in PBS) Fluorophore/Protein Ratio: 1.7 moles IRDye 800CW: 1mole IgG IRDye 800CW antibodies can be used for a variety of applications, including Western blotting, In-Cell Western assays, In-Gel Westerns, and many others. Isolation of specific antibodise was accomplished by immunoaffinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis, this antibody reacts with the heavy chains of rabbit IgG as well as the light chains common to most rabbit immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid phase adsorbed for minimal cross-reactivity with human, mouse, and rat serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western applications. |
免疫原 | Native Rabbit IgG |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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