GAPDH Loading Control Polyclonal Antibody 2 is a Rabbit antibody targeting GAPDH Loading Control. GAPDH Loading Control Polyclonal Antibody 2 can be used in ELISA,IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Rat,Hamster

1. Sample:
Lane 1: Cerebellum (Mouse) Lysate
Lane 2: Raw264.7 (Mouse) Cell Lysate
Lane 3: NIH/3T3 (Mouse) Cell Lysate
Lane 4: Cerebrum (Rat) Lysate
Lane 5: SH-SY5Y (Human) Cell Lysate
Lane 6: MCF-7 (Human) Cell Lysate
Primary: Anti-GAPDH (TMAB-00742) at 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
2. Sample:
Lane 1,4,7: Mouse Cerebellum tissue lysates
Lane 2,5,8: Rat Cerebellum tissue lysates
Lane 3,6,9: Human SH-SY5Y cell lysates
Primary: Anti-GAPDH (TMAB-00742) at 1/10000~1/100000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kDa
Observed band size: 36 kDa
3. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (TMAB-00742) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (TMAB-00742) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (TMAB-00742) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (TMAB-00742) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (TMAB-00742) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (TMAB-00742) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (TMAB-00742) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (TMAB-00742) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (TMAB-00742) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (TMAB-00742) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
13. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (TMAB-00742) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
14. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (TMAB-00742) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
15. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (TMAB-00742) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red), DAPI (blue) was used to stain the cell nucleus.
16. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (TMAB-00742) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red), DAPI (blue) was used to stain the cell nucleus.
17. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (TMAB-00742) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red), DAPI (blue) was used to stain the cell nucleus.
18. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (TMAB-00742) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red), DAPI (blue) was used to stain the cell nucleus.

WB: 1:5000-50000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000

Polyclonal

Recombinant Protein: human GAPDH full length protein

Human

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

Energy Metabolism,Huntington's disease,Alzheimer's disease,Neurodegenerative disease,Cancer,Energy Metabolism,Carbohydrate metabolism,GAPDH,Metabolism of carbohydrates

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:3170585, PubMed:11724794).Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:3170585, PubMed:11724794).Modulates the organization and assembly of the cytoskeleton (By similarity).Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity).Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094).Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094).Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501).Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity).Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).