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GAPDH Loading Control Antibody (9U185)
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产品编号 TMAB-00741
GAPDH Loading Control Antibody (9U185) 是一种 Mouse 抗体，靶向 GAPDH Loading Control。GAPDH Loading Control Antibody (9U185) 可用于 WB。
GAPDH Loading Control Antibody (9U185)
GAPDH Loading Control Antibody (9U185)
还可以产品编号 TMAB-00741
GAPDH Loading Control Antibody (9U185) 是一种 Mouse 抗体，靶向 GAPDH Loading Control。GAPDH Loading Control Antibody (9U185) 可用于 WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 660 | 5日内发货 | |
| 100 μL | ¥ 970 | 5日内发货 | |
| 500 μL | ¥ 3,985 | 5日内发货 |
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产品介绍
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抗原信息
| 产品描述 | GAPDH Loading Control Antibody (9U185) is a Mouse antibody targeting GAPDH Loading Control. GAPDH Loading Control Antibody (9U185) can be used in WB. |
| Ig Type | IgG |
| 克隆号 | 9U185 |
| 交叉反应 | Human,Mouse,Rat,Hamster (predicted:Chicken,Dog,Pig,Rabbit,Sheep,Monkey) |
| 验证活性 | 1. Sample: Lane 1: Human HEK293 cell lysates Lane 2: Human Hela cell lysates Lane 3: Human SH-SY5Y cell lysates Lane 4: Mouse NIH/3T3 cell lysates Lane 5: Hamster CHO cell lysates Lane 6: Rat Brain tissue lysates Primary: Anti-GAPDH (TMAB-00741) at 1/200000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 38 kDa Observed band size: 38 kDa 2. Sample: Lane 1: Rat Cerebrum tissue lysates Lane 2: Rat Heart tissue lysates Lane 3: Human A549 cell lysates Lane 4: Human Jurkat cell lysates Lane 5: Human Huvec cell lysates Lane 6: Human Hela cell lysates Lane 7: Human U2os cell lysates Lane 8: Human HepG2 cell lysates Primary: Anti-GAPDH (TMAB-00741) at 1/50000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 38 kDa Observed band size: 38 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:5000-500000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Postnuclear and Perinuclear regions. |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein G purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | oading Control Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits. |
| 免疫原 | Recombinded Human GAPDH |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Energy Metabolism,Huntington's disease,Alzheimer's disease,Neurodegenerative disease,Cancer,Energy Metabolism,Carbohydrate metabolism,GAPDH,Metabolism of carbohydrates |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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