GAPDH Loading Control Antibody (9U185) is a Mouse antibody targeting GAPDH Loading Control. GAPDH Loading Control Antibody (9U185) can be used in WB.

IgG

9U185

Human,Mouse,Rat,Hamster (predicted:Chicken,Dog,Pig,Rabbit,Sheep,Monkey)

1. Sample:
Lane 1: Human HEK293 cell lysates
Lane 2: Human Hela cell lysates
Lane 3: Human SH-SY5Y cell lysates
Lane 4: Mouse NIH/3T3 cell lysates
Lane 5: Hamster CHO cell lysates
Lane 6: Rat Brain tissue lysates
Primary: Anti-GAPDH (TMAB-00741) at 1/200000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
2. Sample:
Lane 1: Rat Cerebrum tissue lysates
Lane 2: Rat Heart tissue lysates
Lane 3: Human A549 cell lysates
Lane 4: Human Jurkat cell lysates
Lane 5: Human Huvec cell lysates
Lane 6: Human Hela cell lysates
Lane 7: Human U2os cell lysates
Lane 8: Human HepG2 cell lysates
Primary: Anti-GAPDH (TMAB-00741) at 1/50000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa

WB: 1:5000-500000

Monoclonal

Recombinded Human GAPDH

Human

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

Energy Metabolism,Huntington's disease,Alzheimer's disease,Neurodegenerative disease,Cancer,Energy Metabolism,Carbohydrate metabolism,GAPDH,Metabolism of carbohydrates

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:3170585, PubMed:11724794).Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:3170585, PubMed:11724794).Modulates the organization and assembly of the cytoskeleton (By similarity).Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity).Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094).Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094).Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501).Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity).Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).