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F(ab')₂ Fragment Goat Anti-Mouse IgG H&L Secondary Antibody-Biotin

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产品编号 TMAB-02063B
别名 F(ab)2 Fragment Goat Anti-Mouse IgG(H+L)/Biotin, F(ab)2 Fragment Goat Anti-Mouse IgG H&L/Biotin, F(ab)2 Fragment Goat Anti-Mouse IgG H&L (Biotin)

F(ab')₂ Fragment Goat Anti-Mouse IgG H&L Secondary Antibody-Biotin 是一种 Biotin-conjugated Goat 抗体，靶向 IgG H&L。F(ab')₂ Fragment Goat Anti-Mouse IgG H&L Secondary Antibody-Biotin 可用于 ELISA,ICC/IF,IF,IHC-F,IHC-P,WB。

F(ab')₂ Fragment Goat Anti-Mouse IgG H&L Secondary Antibody-Biotin

F(ab')₂ Fragment Goat Anti-Mouse IgG H&L Secondary Antibody-Biotin

一键复制产品信息
Rating icon 还可以
产品编号 TMAB-02063B 别名 F(ab)2 Fragment Goat Anti-Mouse IgG(H+L)/Biotin, F(ab)2 Fragment Goat Anti-Mouse IgG H&L/Biotin, F(ab)2 Fragment Goat Anti-Mouse IgG H&L (Biotin)

F(ab')₂ Fragment Goat Anti-Mouse IgG H&L Secondary Antibody-Biotin 是一种 Biotin-conjugated Goat 抗体，靶向 IgG H&L。F(ab')₂ Fragment Goat Anti-Mouse IgG H&L Secondary Antibody-Biotin 可用于 ELISA,ICC/IF,IF,IHC-F,IHC-P,WB。

规格价格库存数量
100 μL
¥ 415
5日内发货
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产品介绍

生物活性
产品描述
F(ab')₂ Fragment Goat Anti-Mouse IgG H&L Secondary Antibody-Biotin is a Biotin-conjugated Goat antibody targeting IgG H&L. F(ab')₂ Fragment Goat Anti-Mouse IgG H&L Secondary Antibody-Biotin can be used in ELISA,ICC/IF,IF,IHC-F,IHC-P,WB.
别名F(ab)2 Fragment Goat Anti-Mouse IgG(H+L)/Biotin, F(ab)2 Fragment Goat Anti-Mouse IgG H&L/Biotin, F(ab)2 Fragment Goat Anti-Mouse IgG H&L (Biotin)
Ig Type
IgG
交叉反应
Mouse
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GLP-1) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02063B 1:1000) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02063B 1:500) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02063B 1:500) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02063B 1:500) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CK7) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02063B 1:500) instructionsand DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02063B 1:500) instructionsand DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02063B 1:500) instructionsand DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02063B 1:500) instructionsand DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02063B 1:500) instructionsand DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02063B 1:500) instructionsand DAB staining.
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
应用ELISAICC/IFIFIHC-FIHC-PWB
推荐剂量
IHC-P: 1:400-800; WB: 1:1000-20000; IHC-F: 1:100-1000; ELISA: 1:1000-20000; ICC/IF: 1:100-1000; IF: 1:100-1000; FCM: 1:100-1000
抗体种类
Polyclonal
宿主来源Goat
构建方式Polyclonal Antibody
纯化方式Affinity purified by Protein G, nonspecific adsorbed
性状Liquid
缓冲液10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol.
研究背景Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
抗原信息
免疫原
Native Mouse IgG
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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