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顶部生物活性储存和溶解信息计算器

Beta-Actin Loading Control Antibody (9Q394)

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Beta-Actin Loading Control Antibody (9Q394)
产品编号 TMAY-00015

Beta-Actin Loading Control Antibody (9Q394) 是一种 Rabbit 抗体，靶向 Beta-Actin。Beta-Actin Loading Control Antibody (9Q394) 可用于 WB,IP。

Beta-Actin Loading Control Antibody (9Q394)
331

Beta-Actin Loading Control Antibody (9Q394)

Rating icon 还可以
Beta-Actin Loading Control Antibody (9Q394)
产品编号 TMAY-00015

Beta-Actin Loading Control Antibody (9Q394) 是一种 Rabbit 抗体，靶向 Beta-Actin。Beta-Actin Loading Control Antibody (9Q394) 可用于 WB,IP。

规格价格库存数量
20 μL
¥ 395
5日内发货
100 μL
¥ 1,190
5日内发货
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产品介绍

生物活性
产品描述
Beta-Actin Loading Control Antibody (9Q394) is a Rabbit antibody targeting Beta-Actin. Beta-Actin Loading Control Antibody (9Q394) can be used in WB,IP.
Ig Type
Rabbit IgG
克隆号
9Q394
交叉反应
Human
验证活性
1. Anti-beta-Actin rabbit monoclonal antibody at 1:20000 dilution.
-Lane A: Jurkat Whole Cell Lysate.
-Lane B: HeLa Whole Cell Lysate.
-Lane C: HepG2 Whole Cell Lysate.
-Lane D: A549 Whole Cell Lysate.
-Lane E: Mouse brain tissue Lysate.
-Lane F: Mouse skeletal muscle tissue Lysate.
-Lane G: Mouse kidney tissue Lysate.
-Lane H: Rat brain tissue Lysate.
-Lane I: Rat lung tissue Lysate.
-Lane J: Rat kidney tissue lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
-Developed using the ECL technique.
-Performed under reducing conditions.
-Predicted band size:42 kDa.
-Observed band size:42 kDa.
2. Actin was immunoprecipitated using:
-Lane A:0.5 mg HeLa Whole Cell Lysate.
-Lane B:0.5 mg Jurkat Whole Cell Lysate.
-Lane C:0.5 mg MCF7 Whole Cell Lysate.
-Lane D:0.5 mg A431 Whole Cell Lysate.
-4 µL anti-Actin rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.
-Primary antibody:
-Anti-Actin rabbit monoclonal antibody, at 1:100 dilution.
-Secondary antibody:
-Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution.
-Developed using the ECL technique.
-Performed under reducing conditions.
-Predicted band size: 43 kDa.
-Observed band size:44 kDa
应用
WB,IP
推荐剂量
WB: 1:10000-1:100000; IP: 1-5 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Rabbit
构建方式It is a chimeric monoclonal antibody combining the constant domains of the rabbit IgG molecule with mouse variable regions. The variable region was obtained from a mouse immunized with A synthetic peptide corresponding to the N-terminus of the Human beta Actin / ACTB. The antibody was produced using recombinant antibody technology.
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景ACTB (Actin Beta) is a Protein Coding gene. Diseases associated with ACTB include Dystonia, Juvenile-Onset and Baraitser-Winter Syndrome 1. Among its related pathways are Salivary secretion and Development Slit-Robo signaling. Gene Ontology (GO) annotations related to this gene include identical protein binding and RNA polymerase II proximal promoter sequence-specific DNA binding. An important paralog of this gene is ACTG1. TUBB, HPRT and ACTB were the most stably expressed genes for all analysis groups across meningioma and non-pathological meningeal tissue combined. ACTB, a significant upregulated gene in abdominal aortic aneurysm samples, could be regulated by CLIC4, which was significantly enriched in cell motions. Dystonia-deafness syndrome is a well-known clinical entity, with sensorineural deafness typically manifesting earlier than dystonia. ACTB p.Arg183Trp heterozygosity has been reported in six patients to cause combined infant-onset deafness and dystonia manifesting in adolescence or young adulthood.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
A synthetic peptide: N-terminus of the Human beta Actin / ACTB
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

计算器

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关键词

β-actinβactincytoplasmic 1Beta-actinb-actinActin, cytoplasmic1Actin, cytoplasmic 1ACTB

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