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Anti-XRCC5 Antibody (3P286)

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Anti-XRCC5 Antibody (3P286)
产品编号 TMAH-01257

Anti-XRCC5 Antibody (3P286) 是一种抗体,靶向 XRCC5。Anti-XRCC5 Antibody (3P286) 可用于 ELISA, WB, IHC, IF。

Anti-XRCC5 Antibody (3P286)

Anti-XRCC5 Antibody (3P286)

Rating icon 还可以
Anti-XRCC5 Antibody (3P286)
产品编号 TMAH-01257

Anti-XRCC5 Antibody (3P286) 是一种抗体,靶向 XRCC5。Anti-XRCC5 Antibody (3P286) 可用于 ELISA, WB, IHC, IF。

规格价格库存数量
50 μL
¥ 1,315
5日内发货
100 μL
¥ 2,195
5日内发货
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产品介绍

生物活性
产品描述
Anti-XRCC5 Antibody (3P286) is an antibody targeting XRCC5. Anti-XRCC5 Antibody (3P286) can be used in ELISA, WB, IHC, IF.
Ig Type
Rabbit IgG
克隆号
3P286
交叉反应
Human
验证活性
1. Western Blot
-Positive WB detected in: K562 whole cell lysate, A549 whole cell lysate, 293T whole cell lysate
-All lanes: XRCC5 antibody at 1:2000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 83 kDa
-Observed band size: 83 kDa
2. IHC image of TMAH-01257 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
3. IHC image of TMAH-01257 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
4. Immunofluorescence staining of Hela Cells with TMAH-01257 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
应用
推荐剂量
WB:1:500-1:5000; IHC:1:50-1:200; IF:1:20-1:200.
抗体种类
Monoclonal
亚细胞定位Nucleus. Nucleus, nucleolus. Chromosome.
构建方式Recombinant Antibody
纯化方式Affinity-chromatography
性状Liquid
缓冲液Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA. Required for double-strand break repair and V(D)J recombination. Also has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. During NHEJ, the XRCC5-XRRC6 dimer performs the recognition step: it recognizes and binds to the broken ends of the DNA and protects them from further resection. Binding to DNA may be mediated by XRCC6. The XRCC5-XRRC6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5-XRRC6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. The XRCC5-XRRC6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5-XRRC6 dimer together with APEX1 acts as a negative regulator of transcription. In association with NAA15, the XRCC5-XRRC6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. As part of the DNA-PK complex, involved in the early steps of ribosome assembly by promoting the processing of precursor rRNA into mature 18S rRNA in the small-subunit processome. Binding to U3 small nucleolar RNA, recruits PRKDC and XRCC5/Ku86 to the small-subunit processome. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
A synthetic peptide: Human Ku80
抗原种属
Human
基因ID
Uniprot ID
研究领域
Epigenetics and Nuclear Signaling
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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