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Anti-Vinculin Antibody (3Q495)
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产品编号 TMAC-04263
别名 vinculin, MVCL, MV, HEL114, CMH15, CMD1W
Anti-Vinculin Antibody (3Q495) 是一种 Rabbit 抗体，靶向 Vinculin。Anti-Vinculin Antibody (3Q495) 可用于 WB,IHC,ICC/IF。
Anti-Vinculin Antibody (3Q495)
Anti-Vinculin Antibody (3Q495)
还可以产品编号 TMAC-04263 别名 vinculin, MVCL, MV, HEL114, CMH15, CMD1W
Anti-Vinculin Antibody (3Q495) 是一种 Rabbit 抗体，靶向 Vinculin。Anti-Vinculin Antibody (3Q495) 可用于 WB,IHC,ICC/IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,480 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-Vinculin Antibody (3Q495) is a Rabbit antibody targeting Vinculin. Anti-Vinculin Antibody (3Q495) can be used in WB,IHC,ICC/IF. |
| 别名 | vinculin, MVCL, MV, HEL114, CMH15, CMD1W |
| Ig Type | IgG |
| 克隆号 | 3Q495 |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of Vinculin on different lysates with Vinculin antibody at 1/20,000 dilution. Lane 1: HepG2 cell lysate (15 μg/Lane), Lane 2: HeLa cell lysate (15 μg/Lane), Lane 3: NIH/3T3 cell lysate (15 μg/Lane), Lane 4: C6 cell lysate (15 μg/Lane), Lane 5: Mouse spleen tissue lysate (20 μg/Lane), Lane 6: Rat kidney tissue lysate (20 μg/Lane), Predicted band size: 124 kDa, Observed band size: 124 kDa, Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. 2. Immunocytochemistry analysis of HeLa cells labeling Vinculin with Vinculin antibody at 1/1,000 dilution. 3. Flow cytometric analysis of HeLa cells labeling Vinculin. 4. Immunohistochemical analysis of paraffin-embedded human testis tissue with Vinculin antibody at 1/200 dilution. 5. Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Vinculin antibody at 1/20,000 dilution. 6. Immunohistochemical analysis of paraffin-embedded rat colon tissue with Vinculin antibody at 1/20,000 dilution. 7. Immunocytochemistry analysis of NIH/3T3 cells labeling Vinculin with Vinculin antibody at 1/250 dilution. 8. Immunocytochemistry analysis of C6 cells labeling Vinculin with Vinculin antibody at 1/250 dilution. |
| 应用 | |
| 推荐剂量 | WB: 1:5000-20000; ICC/IF-Cell 1:200-1000; ICC/IF-Tissue 1:50-4000; IHC-P 1:200-20000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and α-actinin. In addition, vinculin, Talin and α-actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. α-Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin. |
| 偶联 | Unconjugated |
| 免疫原 | A synthesized peptide: within human Vinculin |
| 抗原种属 | human |
| Uniprot ID |
| 分子量 | Theoretical: 124 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months (Store at 4℃ after thawing). Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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