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Anti-USP22 Antibody (1F346) 是一种 Rabbit 抗体,靶向 USP22。Anti-USP22 Antibody (1F346) 可用于 WB,ICC/IF,FCM,IP。
Anti-USP22 Antibody (1F346) 是一种 Rabbit 抗体,靶向 USP22。Anti-USP22 Antibody (1F346) 可用于 WB,ICC/IF,FCM,IP。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,480 | 5日内发货 | |
100 μL | ¥ 2,480 | 5日内发货 |
产品描述 | Anti-USP22 Antibody (1F346) is a Rabbit antibody targeting USP22. Anti-USP22 Antibody (1F346) can be used in WB,ICC/IF,FCM,IP. |
别名 | USP3L, USP 22, Ubiquitin-specific-processing protease 22, Ubiquitin thioesterase 22, Ubiquitin carboxyl-terminal hydrolase 22, KIAA1063, EC 3.4.19.12, Deubiquitinating enzyme 22 |
Ig Type | IgG |
交叉反应 | Human |
验证活性 | 1. Western blot analysis of USP22 on Hela (1) and HepG2 (2) cell lysate using anti-USP22 antibody at 1/500 dilution. 2. ICC staining USP22 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining USP22 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining USP22 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of Hela cells with USP22 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:1000-5000; ICC/IF: 1:50-200; IP: 1:10-50; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP22 (ubiquitin specific peptidase 22), also known as USP3L, is a 525 amino acid protein that contains one UBP-type zinc finger and functions to catalyze the conversion of a ubiquitin C-terminal thioester to free ubiquitin and thiol, a reaction that may influence several cellular processes. Via its catalytic activity, USP22 is thought to play an important role in cell cycle progression and may also serve as a cancer stem cell marker. The gene encoding USP22 maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 60 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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