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Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1K888)
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产品编号 TMAY-02188
别名 ubiquitin-like modifier activating enzyme 1, UBE1X, UBE1, UBA1A, SMAX2, POC20, GXP1, CTD-2522E6.1, CFAP124, AMCX1, A1ST, A1S9T, A1S9
Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1K888) 是一种 Mouse 抗体，靶向 Ubiquitin Activating Enzyme E1/UBA1。Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1K888) 可用于 ELISA,IP。
Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1K888)
331 Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1K888)
还可以产品编号 TMAY-02188 别名 ubiquitin-like modifier activating enzyme 1, UBE1X, UBE1, UBA1A, SMAX2, POC20, GXP1, CTD-2522E6.1, CFAP124, AMCX1, A1ST, A1S9T, A1S9
Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1K888) 是一种 Mouse 抗体，靶向 Ubiquitin Activating Enzyme E1/UBA1。Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1K888) 可用于 ELISA,IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 100 μL | ¥ 1,694 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1K888) is a Mouse antibody targeting Ubiquitin Activating Enzyme E1/UBA1. Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1K888) can be used in ELISA,IP. |
| 别名 | ubiquitin-like modifier activating enzyme 1, UBE1X, UBE1, UBA1A, SMAX2, POC20, GXP1, CTD-2522E6.1, CFAP124, AMCX1, A1ST, A1S9T, A1S9 |
| Ig Type | Mouse IgG1 |
| 克隆号 | 1K888 |
| 交叉反应 | Human |
| 验证活性 | UBA1 was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg K562 Whole Cell Lysate -0.5 µL anti-UBA1 mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein G. -Primary antibody: -Anti-UBA1 mouse monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 111 kDa. -Observed band size: 111 kDa |
| 应用 | ELISA,IP |
| 推荐剂量 | ELISA: 1:5000-1:10000; IP: 0.2-1 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human UBE1 / UBA1 (rh UBE1 / UBA1; TMPY-02840; NP_003325.2; Ser2-Arg1058). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS with 10% Trehalose, pH7.0 |
| 研究背景 | UBE1, also known as UBA1, belongs to the ubiquitin-activating E1 family. UBE1 gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. It also catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Defects in UBA1 can cause spinal muscular atrophy X-linked type 2 (SMAX2), also known as X-linked lethal infantile spinal muscular atrophy, distal X-linked arthrogryposis multiplex congenita or X-linked arthrogryposis type 1 (AMCX1). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX2 is a lethal infantile form presenting with hypotonia, areflexia, and multiple congenital contractures. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein: Human UBE1 / UBA1 protein (TMPY-02840) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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