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顶部生物活性储存和溶解信息计算器

Anti-U1A Antibody (7N374)

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Anti-U1A Antibody (7N374)
产品编号 TMAC-04182
别名 zgc:77810, wu:fc19d01, U1-A, U1A, U1 snRNP specific protein A, U1 snRNP A, U1 small nuclear RNP specific A, U1 small nuclear ribonucleoprotein A, SNRPA_HUMAN, SNRPA, snRNP protein A, snRNP A, small nuclear ribonucleoprotein polypeptide A, Mud1, fc19d01

Anti-U1A Antibody (7N374) 是一种 Rabbit 抗体，靶向 U1A。Anti-U1A Antibody (7N374) 可用于 WB,ICC,IF,IHC,IP。

Anti-U1A Antibody (7N374)

Anti-U1A Antibody (7N374)

Rating icon 还可以
Anti-U1A Antibody (7N374)
产品编号 TMAC-04182 别名 zgc:77810, wu:fc19d01, U1-A, U1A, U1 snRNP specific protein A, U1 snRNP A, U1 small nuclear RNP specific A, U1 small nuclear ribonucleoprotein A, SNRPA_HUMAN, SNRPA, snRNP protein A, snRNP A, small nuclear ribonucleoprotein polypeptide A, Mud1, fc19d01

Anti-U1A Antibody (7N374) 是一种 Rabbit 抗体，靶向 U1A。Anti-U1A Antibody (7N374) 可用于 WB,ICC,IF,IHC,IP。

规格价格库存数量
50 μL
¥ 1,480
5日内发货
100 μL
¥ 2,480
5日内发货
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产品介绍

生物活性
产品描述
Anti-U1A Antibody (7N374) is a Rabbit antibody targeting U1A. Anti-U1A Antibody (7N374) can be used in WB,ICC,IF,IHC,IP.
别名zgc:77810, wu:fc19d01, U1-A, U1A, U1 snRNP specific protein A, U1 snRNP A, U1 small nuclear RNP specific A, U1 small nuclear ribonucleoprotein A, SNRPA_HUMAN, SNRPA, snRNP protein A, snRNP A, small nuclear ribonucleoprotein polypeptide A, Mud1, fc19d01
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of U1A on different lysates using anti-U1A antibody at 1/500 dilution. Positive control: Lane 1: Rat brain, Lane 2: K562, Lane 3: A431, Lane 4: Mouse kidney.
2. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-U1A antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-U1A antibody. Counter stained with hematoxylin.
4. ICC staining U1A in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining U1A in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining U1A in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
应用
WB
ICC
IF
IHC
IP
推荐剂量
WB: 1:500-1000; IHC: 1:50-200; ICC: 1:50-200; IP: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景SNRPA (small nuclear ribonucleoprotein polypeptide A), also known as U1A (U1 snRNP protein A), is a component of the RNA spliceosome, a complex of proteins that are required for the precise excision of introns from pre-messenger RNA (pre-mRNA). Localizing to the nucleus, SNRPA contains two RRM (RNA recognition motif) domains, namely RRM1 and RRM2, and RRM1 specifically associates with the stem loop II of U1 snRNA (small nuclear RNA). In addition to functioning as a component of the U1 snRNP, SNRPA negatively regulates polyadenylation of SNRPA pre-mRNA, thereby negatively regulating itself. By inhibiting the addition of a polyA tail that would allow the pre-mRNA to mature, SNRPA causes the nuclear exosome degradation of the SNRPA pre-mRNA. At least 16% of cellular SNRPA also functions in an snRNP-free form (SF-A) that complexes with a group of non-snRNP proteins.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: N-terminal human U1A
抗原种属
human
Uniprot ID
P09012
化学信息
分子量Theoretical: 31 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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