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顶部生物活性储存和溶解信息计算器

Anti-TOP2A Antibody (1C511)

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产品编号 TMAH-01198

别名 TOP2, EC 5.99.1.3, DNA topoisomerase II, DNA topoisomerase 2-alpha, alpha isozyme

Anti-TOP2A Antibody (1C511) 是一种抗体，靶向 TOP2A。Anti-TOP2A Antibody (1C511) 可用于 ELISA,IHC,WB。

Anti-TOP2A Antibody (1C511)

Anti-TOP2A Antibody (1C511)

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Rating icon 还可以
产品编号 TMAH-01198 别名 TOP2, EC 5.99.1.3, DNA topoisomerase II, DNA topoisomerase 2-alpha, alpha isozyme

Anti-TOP2A Antibody (1C511) 是一种抗体，靶向 TOP2A。Anti-TOP2A Antibody (1C511) 可用于 ELISA,IHC,WB。

规格价格库存数量
50 μL
¥ 1,310
5日内发货
100 μL
¥ 2,190
5日内发货
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产品介绍

生物活性
产品描述
Anti-TOP2A Antibody (1C511) is an antibody targeting TOP2A. Anti-TOP2A Antibody (1C511) can be used in ELISA,IHC,WB.
别名TOP2, EC 5.99.1.3, DNA topoisomerase II, DNA topoisomerase 2-alpha, alpha isozyme
Ig Type
Rabbit IgG
克隆号
1C511
交叉反应
Human, Mouse
验证活性
1. Western Blot
-Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, NIH/3T3 whole cell lysate
-All lanes: TOP2A antibody at 1:1500
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 175, 178, 179, 183 kDa
-Observed band size: 175 kDa
2. IHC image of TMAH-01198 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
应用ELISAIHCWB
推荐剂量
WB:1:500-1:5000; IHC:1:50-1:200.
抗体种类
Monoclonal
亚细胞定位Cytoplasm. Nucleus, nucleoplasm. Nucleus. Nucleus, nucleolus.
组织特异性Expressed in the tonsil, spleen, lymph node, thymus, skin, pancreas, testis, colon, kidney, liver, brain and lung. Also found in high-grade lymphomas, squamous cell lung tumors and seminomas.
构建方式Recombinant Antibody
纯化方式Affinity-chromatography
性状Liquid
缓冲液Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
A synthetic peptide: Human Topoisomerase II alpha
抗原种属
Human
基因名称
TOP2A
基因ID
7153
Uniprot ID
P11388
研究领域
Epigenetics and Nuclear Signaling, Cancer
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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