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Anti-Strep Tag II Antibody (4T542)

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Anti-Strep Tag II Antibody (4T542)
产品编号 TMAB-01794

Anti-Strep Tag II Antibody (4T542) 是一种 Mouse 抗体，靶向 Strep Tag II。Anti-Strep Tag II Antibody (4T542) 可用于 WB。

Anti-Strep Tag II Antibody (4T542)

Anti-Strep Tag II Antibody (4T542)

Rating icon 还可以
Anti-Strep Tag II Antibody (4T542)
产品编号 TMAB-01794

Anti-Strep Tag II Antibody (4T542) 是一种 Mouse 抗体，靶向 Strep Tag II。Anti-Strep Tag II Antibody (4T542) 可用于 WB。

规格价格库存数量
50 μL
¥ 1,165
5日内发货
100 μL
¥ 1,970
5日内发货
200 μL
¥ 2,795
5日内发货
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产品介绍

生物活性
产品描述
Anti-Strep Tag II Antibody (4T542) is a Mouse antibody targeting Strep Tag II. Anti-Strep Tag II Antibody (4T542) can be used in WB.
Ig Type
IgG
克隆号
4T542
交叉反应
Species independent
验证活性
1. Sample:
Strep-Tagged Fusion Protein Overexpression E.coli Lysate at 4 μg
Primary: Anti-Strep-Tag II (TMAB-01794) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
2. Sample:
Lane 1: Strep-Tagged Fusion Protein Overexpression E.coli Lysates
Lane 2: Negative control
Primary: Anti-Strep-Tag II/HRP (TMAB-01794-HRP) at 1/10000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
应用
WB
推荐剂量
WB: 1:1000-5000
抗体种类
Monoclonal
宿主来源Mouse
构建方式Hybridoma Monoclonal Antibody
纯化方式Protein G purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景The Strep-tag system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins.
抗原信息
免疫原
KLH conjugated synthetic peptide Strep-Tag
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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