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Anti-SP1 Antibody (3K908)
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产品编号 TMAC-03833
别名 TSFP1, TSFP 1, Transcription factor Sp1, Specificity protein 1, Sp1 transcription factor isoform a, SP 1
Anti-SP1 Antibody (3K908) 是一种 Rabbit 抗体，靶向 SP1。Anti-SP1 Antibody (3K908) 可用于 WB,ICC/IF,IHC。
Anti-SP1 Antibody (3K908)
Anti-SP1 Antibody (3K908)
还可以产品编号 TMAC-03833 别名 TSFP1, TSFP 1, Transcription factor Sp1, Specificity protein 1, Sp1 transcription factor isoform a, SP 1
Anti-SP1 Antibody (3K908) 是一种 Rabbit 抗体，靶向 SP1。Anti-SP1 Antibody (3K908) 可用于 WB,ICC/IF,IHC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-SP1 Antibody (3K908) is a Rabbit antibody targeting SP1. Anti-SP1 Antibody (3K908) can be used in WB,ICC/IF,IHC. |
| 别名 | TSFP1, TSFP 1, Transcription factor Sp1, Specificity protein 1, Sp1 transcription factor isoform a, SP 1 |
| Ig Type | IgG |
| 交叉反应 | Human |
| 验证活性 | 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-SP1 antibody. Counter stained with hematoxylin. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SP1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SP1 antibody. Counter stained with hematoxylin. 4. ICC staining SP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining SP1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining SP1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:100-500 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Sp1 is a sequence-specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 was initially identified as a HeLa cell-derived factor that selectively activates in vitro transcription from the SV40 promoter and binds to the multiple GC boxes in the 21-bp repeated elements in SV40. The sequence specificity of DNA binding is conferred by Zn (II) fingers, whereas a different region of Sp1 appears to regulate the affinity of DNA binding. Sp1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences. Evidence suggests that the early growth response gene, Erg-1 (also known as Zif268 or NGF1-A), may downregulate certain mammalian gene promoters by competing with Sp1 for binding to an overlapping binding motif. The gene encoding human Sp1 maps to chromosome 12q13.1. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 81 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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