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Anti-SAE1 Antibody (3S832)
还可以产品编号 TMAH-01069
别名 UBLE1A, SUMO1 activating enzyme subunit 1, SUA1, HSPC140, AOS1
Anti-SAE1 Antibody (3S832) 是一种抗体，靶向 SAE1。Anti-SAE1 Antibody (3S832) 可用于 ELISA, WB, IF。
Anti-SAE1 Antibody (3S832)
Anti-SAE1 Antibody (3S832)
还可以产品编号 TMAH-01069 别名 UBLE1A, SUMO1 activating enzyme subunit 1, SUA1, HSPC140, AOS1
Anti-SAE1 Antibody (3S832) 是一种抗体，靶向 SAE1。Anti-SAE1 Antibody (3S832) 可用于 ELISA, WB, IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,300 | 5日内发货 | |
| 100 μL | ¥ 2,190 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-SAE1 Antibody (3S832) is an antibody targeting SAE1. Anti-SAE1 Antibody (3S832) can be used in ELISA, WB, IF. |
| 别名 | UBLE1A, SUMO1 activating enzyme subunit 1, SUA1, HSPC140, AOS1 |
| Ig Type | Rabbit IgG |
| 克隆号 | 3S832 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in: K562 whole cell lysate -All lanes: SAE1 Antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 39, 30, 34 kDa -Observed band size: 39 kDa 2. Immunofluorescence staining of Hela Cells with TMAH-01069 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| 应用 | ELISA, WB, IF |
| 推荐剂量 | WB:1:500-1:5000; IF:1:20-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Nucleus. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human SAE1 |
| 抗原种属 | Human |
| 基因ID | 10055 |
| Uniprot ID | |
| 研究领域 | Cell biology |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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