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顶部生物活性储存和溶解信息计算器

Anti-SAE1 Antibody (3S832)

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产品编号 TMAH-01069

别名 UBLE1A, SUMO1 activating enzyme subunit 1, SUA1, HSPC140, AOS1

Anti-SAE1 Antibody (3S832) 是一种抗体，靶向 SAE1。Anti-SAE1 Antibody (3S832) 可用于 ELISA,IF,WB。

Anti-SAE1 Antibody (3S832)

Anti-SAE1 Antibody (3S832)

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Rating icon 还可以
产品编号 TMAH-01069 别名 UBLE1A, SUMO1 activating enzyme subunit 1, SUA1, HSPC140, AOS1

Anti-SAE1 Antibody (3S832) 是一种抗体，靶向 SAE1。Anti-SAE1 Antibody (3S832) 可用于 ELISA,IF,WB。

规格价格库存数量
50 μL
¥ 1,300
5日内发货
100 μL
¥ 2,190
5日内发货
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产品介绍

生物活性
产品描述
Anti-SAE1 Antibody (3S832) is an antibody targeting SAE1. Anti-SAE1 Antibody (3S832) can be used in ELISA,IF,WB.
别名UBLE1A, SUMO1 activating enzyme subunit 1, SUA1, HSPC140, AOS1
Ig Type
Rabbit IgG
克隆号
3S832
交叉反应
Human
验证活性
1. Western Blot
-Positive WB detected in: K562 whole cell lysate
-All lanes: SAE1 Antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 39, 30, 34 kDa
-Observed band size: 39 kDa
2. Immunofluorescence staining of Hela Cells with TMAH-01069 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
应用ELISAIFWB
推荐剂量
WB:1:500-1:5000; IF:1:20-1:200.
抗体种类
Monoclonal
亚细胞定位Nucleus.
组织特异性Expression level increases during S phase and drops in G2 phase (at protein level).
构建方式Recombinant Antibody
纯化方式Affinity-chromatography
性状Liquid
缓冲液Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
A synthetic peptide: Human SAE1
抗原种属
Human
基因名称
SAE1
基因ID
10055
Uniprot ID
Q9UBE0
研究领域
Cell biology
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

  • 稀释 计算器

关键词

SUA-1SUA 1HSPC-140HSPC 140AOS-1AOS 1

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