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Anti-PSIP1 Antibody (7I174) 是一种 Mouse 抗体,靶向 PSIP1。Anti-PSIP1 Antibody (7I174) 可用于 WB,ICC,IHC。
Anti-PSIP1 Antibody (7I174) 是一种 Mouse 抗体,靶向 PSIP1。Anti-PSIP1 Antibody (7I174) 可用于 WB,ICC,IHC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,995 | 5日内发货 | |
100 μL | ¥ 2,995 | 5日内发货 |
产品描述 | Anti-PSIP1 Antibody (7I174) is a Mouse antibody targeting PSIP1. Anti-PSIP1 Antibody (7I174) can be used in WB,ICC,IHC. |
别名 | Transcriptional coactivator p75/p52, PSIP2, PSIP1, PC4 and SFRS1-interacting protein, Lens epithelium-derived growth factor, LEDGF, DFS70, Dense fine speckles 70 kDa protein (DFS 70), CLL-associated antigen KW-7 |
交叉反应 | Human,Monkey,Rat |
验证活性 | 1. Western blot analysis of PSIP1 on different cell lysates using anti-PSIP1 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2, Lane 2: Jurkat, Lane 3: K562, Lane 4: Cos7, Lane 5: PC-12, Lane 6: Hela, Lane 7: NIH/3T3. 2. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PSIP1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded brain tissue using anti-PSIP1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-PSIP1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-PSIP1 antibody. Counter stained with hematoxylin. 6. ICC staining PSIP1 (green) and actin filaments (red) in NIH/3T3 cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
应用 | |
推荐剂量 | WB: 1:500-1000; IHC: 1:100-500; ICC: 1:100-500 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | Hybridoma Monoclonal Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
研究背景 | PSIP1 (PC4 and SFRS1 interacting protein 1), also known as CLL-associated antigen KW-7, PSIP2, LEDGF (lens epithelium-derived growth factor), PAIP, DFS70 (dense fine speckles 70 kDa protein) or transcriptional coactivator p75/p52, is a 530 amino acid nuclear protein that associates with chromatin throughout the cell cycle. Functioning as a transcriptional coactivator that complexes with the human immunodeficiency virus type 1 (HIV-1) integrase, PSIP1 is essential for the nuclear localization and chromosomal association of viral proteins. As the primary integrase-to-chromatin tethering factor for HIV-1, PSIP1 is responsible for the cellular trafficking of lentiviral integrases. During apoptosis, PSIP1 is cleaved at three sites by caspase-3 and caspase-7, contributing to the pathogenesis of atopic disorders. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 75 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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