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Anti-PPM1G Antibody (3C482)

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产品编号 TMAY-01796

Anti-PPM1G Antibody (3C482) 是一种 Rabbit 抗体,靶向 PPM1G。Anti-PPM1G Antibody (3C482) 可用于 WB,IHC-P,ICC/IF。

Anti-PPM1G Antibody (3C482)

Anti-PPM1G Antibody (3C482)

Rating icon 还可以
产品编号 TMAY-01796

Anti-PPM1G Antibody (3C482) 是一种 Rabbit 抗体,靶向 PPM1G。Anti-PPM1G Antibody (3C482) 可用于 WB,IHC-P,ICC/IF。

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产品介绍

生物活性
产品描述
Anti-PPM1G Antibody (3C482) is a Rabbit antibody targeting PPM1G. Anti-PPM1G Antibody (3C482) can be used in WB,IHC-P,ICC/IF.
Ig Type
Monoclonal Rabbit IgG
克隆号
3C482
交叉反应
Human
验证活性
1. Immunochemical staining of human PPM1G in human heart with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to cardiac muscle.

2. Immunochemical staining of human PPM1G in human epididymis with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to skeletal muscle.

3. Immunofluorescence staining of Human PPM1G in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PPM1G monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).

4. Anti-PPM1G rabbit monoclonal antibody at 1:500 dilution.

-Lane A: Hela Whole Cell Lysate.

-Lane B: 293T Whole Cell Lysate.

-Lane C: Jurkat Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:59 kDa
应用
WB,IHC-P,ICC/IF
推荐剂量
WB: 1:500-1:2000; IHC-P: 1:100-1:500; ICC-IF: 1:20-1:100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human PPM1G (rh PPM1G; TMPY-02197; O15355; Met317-Asp546).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景Protein phosphatase 1G, also known as Protein phosphatase 1C, Protein phosphatase 2C isoform gamma, Protein phosphatase magnesium-dependent 1 gamma, PP2C-gamma, PPM1G and PPM1C, is a cytoplasm protein that belongs to the PP2C family. PPM1G / PP2C-gamma is widely expressed. It is most abundant in testis, skeletal muscle, and heart. Alternatively spliced transcript variants encoding the same protein have been described. PP2C family members are known to be negative regulators of cell stress response pathways. PPM1G / PP2C-gamma is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. PPM1G / PP2C-gamma also plays a role in regulating cell cycle progression.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Human PPM1G protein (TMPY-02197)
抗原种属
Human
研究领域
Phosphatases and Regulators
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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