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顶部生物活性储存和溶解信息计算器

Anti-PHPT1 Antibody (9K764)

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Anti-PHPT1 Antibody (9K764)
产品编号 TMAY-02360
别名 PHP14, phosphohistidine phosphatase 1, HSPC141, HEL-S-132P, CGI-202

Anti-PHPT1 Antibody (9K764) 是一种 Rabbit 抗体，靶向 PHPT1。Anti-PHPT1 Antibody (9K764) 可用于 WB,IP。

Anti-PHPT1 Antibody (9K764)

Anti-PHPT1 Antibody (9K764)

Rating icon 还可以
Anti-PHPT1 Antibody (9K764)
产品编号 TMAY-02360 别名 PHP14, phosphohistidine phosphatase 1, HSPC141, HEL-S-132P, CGI-202

Anti-PHPT1 Antibody (9K764) 是一种 Rabbit 抗体，靶向 PHPT1。Anti-PHPT1 Antibody (9K764) 可用于 WB,IP。

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100 μL
¥ 2,279
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生物活性
产品描述
Anti-PHPT1 Antibody (9K764) is a Rabbit antibody targeting PHPT1. Anti-PHPT1 Antibody (9K764) can be used in WB,IP.
别名PHP14, phosphohistidine phosphatase 1, HSPC141, HEL-S-132P, CGI-202
Ig Type
Rabbit IgG
克隆号
9K764
交叉反应
Human
验证活性
1. Anti-PHPT1 rabbit monoclonal antibody at 1:500 dilution.
-Lane A: HepG2 Whole Cell Lysate.
-Lane B: THP1 Whole Cell Lysate.
-Lane C: MCF7 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:14 kDa.
-Observed band size:14 kDa.
2. PHPT1 was immunoprecipitated using:
-Lane A:0.5 mg HepG2 Whole Cell Lysate.
-4 µL anti-PHPT1 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.
-Primary antibody:
-Anti-PHPT1 rabbit monoclonal antibody, at 1:100 dilution.
-Secondary antibody:
-Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
-Developed using the ECL technique.
-Performed under reducing conditions.
-Predicted band size: 14 kDa.
-Observed band size:14 kDa
应用
WB
IP
推荐剂量
WB: 1:500-1:2000; IP: 1-5 µL/mg of lysate
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human PHPT1 (rh PHPT1; TMPY-02002; Q9NRX4-1; Ala2-Tyr125).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景PHPT1, also known as 14 kDa phosphohistidine phosphatase, phosphohistidine phosphatase 1, protein janus-A homolog, PHP14, is a cytoplasm protein which belongs to the janus family. PHPT1 / PHP14 is expressed abundantly in heart and skeletal muscle. Phosphatases are a diverse group of enzymes that regulate numerous cellular processes. Much of what is known relates to the tyrosine, threonine, and serine phosphatases, whereas the histidine phosphatases have not been studied as much. Protein histidine phosphorylation exists widely in vertebrates, and it plays important roles in signal transduction and other cellular functions. Protein histidine phosphorylation accounts for about 6% of the total protein phosphorylation in eukaryotic cells. The knowledge about eukaryotic PHPT (protein histidine phosphatase) is still very limited. To date, only one vertebrate PHPT has been discovered, and two crystal structures of human PHPT1 have been solved. PHPT1 / PHP14 can dephosphorylate a variety of proteins (e.g. ATP-citrate lyase and the beta-subunit of G proteins). A putative active site has been identified by its electrostatic character, ion binding, and conserved protein residues.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Human PHPT1 protein (TMPY-02002)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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关键词

PHP-14PHP 14HSPC-141HSPC 141CGI202CGI 202

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