Anti-Phospho-YES1 (Tyr426) Polyclonal Antibody is a Rabbit antibody targeting Phospho-YES1 (Tyr426). Anti-Phospho-YES1 (Tyr426) Polyclonal Antibody can be used in WB.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Rabbit,GuineaPig)

1. Sample:
Embryo (Mouse) Lysate at 40 μg
Lung (Mouse) Lysate at 40 μg
BV2 (Human) Cell Lysate at 30 μg
Primary: Anti-phospho-YES1 (Tyr426) (TMAB-01533) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 65 kDa
2. Sample:
Lane 1: Mouse Raw264.7 cell lysates
Lane 2: Rat Embryo tissue lysates
Primary: Anti-phospho-YES1 (Tyr426) (TMAB-01533) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa

WB: 1:500-2000

Polyclonal

KLH conjugated Synthesised phosphopeptide: human YES1 around the phosphorylation site of Tyr426

Human

YES1

Tyrosine-protein kinase Yes

Signal transducers,Proto-oncogenes,Src Family

Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis.