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Anti-Phospho-TrkA (Tyr490) Polyclonal Antibody
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产品编号 TMAB-01525
Anti-Phospho-TrkA (Tyr490) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-TrkA (Tyr490)。Anti-Phospho-TrkA (Tyr490) Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,FCM。
Anti-Phospho-TrkA (Tyr490) Polyclonal Antibody
Anti-Phospho-TrkA (Tyr490) Polyclonal Antibody
还可以产品编号 TMAB-01525
Anti-Phospho-TrkA (Tyr490) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-TrkA (Tyr490)。Anti-Phospho-TrkA (Tyr490) Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,165 | 5日内发货 | |
| 100 μL | ¥ 1,965 | 5日内发货 | |
| 200 μL | ¥ 2,785 | 5日内发货 |
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抗原信息
| 产品描述 | Anti-Phospho-TrkA (Tyr490) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TrkA (Tyr490). Anti-Phospho-TrkA (Tyr490) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat (predicted:Chicken,Horse,Sheep) |
| 验证活性 | 1. Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 μg Lane 2: Cerebrum (Rat) Lysate at 40 μg Primary: Anti-phospho-TrkA (Tyr490) (TMAB-01525) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kDa Observed band size: 130 kDa 2. Blank control (blue): RSC96 cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-phospho-TrkA (Tyr490) antibody (TMAB-01525), Dilution: 0.2 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. 3. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-TrkA (Tyr490)) Polyclonal Antibody, Unconjugated (TMAB-01525) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-TrkA (Tyr490)) Polyclonal Antibody, Unconjugated (TMAB-01525) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cell membrane; Single-pass type I membrane protein. Early endosome membrane; Single-pass type I membrane protein. Note=Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | The Trk family of nerve growth factor receptors includes Trk A(also referfed to as Trk A gp140),Trk B and Trk C. The prototype member of this gene family, Trk A, encodes a 140 kDa cell surface receptor , gp140, the expression of which is restricted in vivo to neurons of the sensory spinal and cranial gangliaof neurocrest origin. Nerve growth factor (NGF) stimulates tyrosine phosphorylation of Trk gp 140 in neural cell lines and in embryonic dorsal root ganglia. By comparison, BDNF and to a lesser extent, NT-3, but not NGF, can induce tyrosine phophorylayion of Trk B gp 145. The third member of the Trk receptor family, Trk C incodes a 140 kDa protein, Trk C gp140, that is preferentially expressed in brain tissue and primarily functions as a receptor for NT-3.An additional component of the Trk receptor complex, NGFR p175, binds to neurotrophic factors with low affinity but is required for efficient signaling. NGFR p175 accelerates Trk activation and may recruit downstream dffector molecules to the ligand-bound receptor complex. |
| 免疫原 | KLH conjugated synthesised phosphopeptide: human TrkA isoform 1(790aa) around the phosphorylation site of Tyr490 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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