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Anti-Phospho-TBK1 (Ser172) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-TBK1 (Ser172)。Anti-Phospho-TBK1 (Ser172) Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-Phospho-TBK1 (Ser172) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-TBK1 (Ser172)。Anti-Phospho-TBK1 (Ser172) Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,170 | 5日内发货 | |
100 μL | ¥ 1,975 | 5日内发货 |
产品描述 | Anti-Phospho-TBK1 (Ser172) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TBK1 (Ser172). Anti-Phospho-TBK1 (Ser172) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
交叉反应 | Rat,Mouse,Human |
验证活性 | 1. Sample: Lane 1: Human Raji cell lysates Lane 2: Human HeLa cell lysates Primary: Anti-Phospho-TBK1 (Ser172) (TMAB-01521) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 84 kDa Observed band size: 84 kDa 2. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-TBK1 (Ser172) Polyclonal Antibody, Unconjugated (TMAB-01521) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-TBK1 (Ser172) Polyclonal Antibody, Unconjugated (TMAB-01521) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-TBK1 (Ser172) Polyclonal Antibody, Unconjugated (TMAB-01521) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-TBK1 (Ser172) Polyclonal Antibody, Unconjugated (TMAB-01521) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-TBK1 (Ser172) Polyclonal Antibody, Unconjugated (TMAB-01521) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Nucleus. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. [provided by RefSeq, Oct 2010] |
免疫原 | KLH conjugated Synthesised phosphopeptide: human TBK1 around the phosphorylation site of Ser172 |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | NFkB pathway,Other Kinases,NFkB Pathway |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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