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Anti-Phospho-Syk (Tyr525, 526) Polyclonal Antibody
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产品编号 TMAB-01514
Anti-Phospho-Syk (Tyr525, 526) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-Syk (Tyr525, 526)。Anti-Phospho-Syk (Tyr525, 526) Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-Phospho-Syk (Tyr525, 526) Polyclonal Antibody
Anti-Phospho-Syk (Tyr525, 526) Polyclonal Antibody
还可以产品编号 TMAB-01514
Anti-Phospho-Syk (Tyr525, 526) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-Syk (Tyr525, 526)。Anti-Phospho-Syk (Tyr525, 526) Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,175 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,790 | 5日内发货 |
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产品介绍
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抗原信息
| 产品描述 | Anti-Phospho-Syk (Tyr525, 526) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Syk (Tyr525, 526). Anti-Phospho-Syk (Tyr525, 526) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Rabbit,GuineaPig) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Syk (Tyr525 + Tyr526)) Polyclonal Antibody, Unconjugated (TMAB-01514) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane1: Embryo (Mouse) Lysate at 30 μg Lane2: Thymus (Mouse) Cell Lysate at 30 μg Primary: Anti-phospho-Syk (Tyr525/526) (TMAB-01514) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72 kDa Observed band size: 70 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cell membrane. Cytoplasm, cytosol. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010] |
| 免疫原 | KLH conjugated Synthesised phosphopeptide: human Syk around the phosphorylation site of Tyr525/526 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Platelets,Mast Cells,Tyrosine Kinases |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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