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Anti-Phospho-SMAD5 (S463, 465) Antibody (2F385) 是一种 Rabbit 抗体,靶向 Phospho-SMAD5 (S463, 465)。Anti-Phospho-SMAD5 (S463, 465) Antibody (2F385) 可用于 WB,ICC,IHC。
Anti-Phospho-SMAD5 (S463, 465) Antibody (2F385) 是一种 Rabbit 抗体,靶向 Phospho-SMAD5 (S463, 465)。Anti-Phospho-SMAD5 (S463, 465) Antibody (2F385) 可用于 WB,ICC,IHC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,490 | 5日内发货 | |
100 μL | ¥ 2,490 | 5日内发货 |
产品描述 | Anti-Phospho-SMAD5 (Ser463, 465) Antibody (2F385) 是一种 Rabbit 抗体,靶向 Phospho-SMAD5 (Ser463, 465)。Anti-Phospho-SMAD5 (Ser463, 465) Antibody (2F385) 可用于 WB,ICC,IHC。 |
别名 | SMAD5 (p-Ser463, 465), SMAD5 (p-S463, 465), SMAD family member 5, SMAD 5, SMAD, SMA and MAD related protein 5, p-SMAD5 (Ser463, 465), p-SMAD5 (S463, 465), Phospho-SMAD5 (S463, 465), MusMLP, mothers against DPP homolog 5, Mothers against DPP homolog 5, mothers against decapentaplegic homolog 5, MADH5, MADH 5, MAD homolog 5, MAD, JV5 1, hSmad5, Dwfc, DKFZp781O1323, DKFZp781C1895 |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of Phospho-Smad5 (S463/465) on different lysates using anti-Phospho-Smad5 (S463/465) antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse brain, Lane 2: Rat brain. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Smad5 (S463/465) antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Phospho-Smad5 (S463/465) antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-Smad5 (S463/465) antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-Smad5 (S463/465) antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Phospho-Smad5 (S463/465) antibody. Counter stained with hematoxylin. 7. ICC staining Phospho-Smad5 (S463/465) in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining Phospho-Smad5 (S463/465) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
应用 | |
推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC: 1:50-200 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | A synthesized phosphopeptide: human Smad5 around the phosphorylation site of Ser463 and 465 |
抗原种属 | human |
Uniprot ID |
分子量 | Theoretical: 58 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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