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Anti-Phospho-RPA2 (Thr21) Antibody (7P562)
还可以产品编号 TMAH-00958
别名 Phospho-RPA2 (T21)
Anti-Phospho-RPA2 (Thr21) Antibody (7P562) 是一种抗体，靶向 Phospho-RPA2 (Thr21)。Anti-Phospho-RPA2 (Thr21) Antibody (7P562) 可用于 ELISA, IF。
Anti-Phospho-RPA2 (Thr21) Antibody (7P562)
Anti-Phospho-RPA2 (Thr21) Antibody (7P562)
还可以产品编号 TMAH-00958 别名 Phospho-RPA2 (T21)
Anti-Phospho-RPA2 (Thr21) Antibody (7P562) 是一种抗体，靶向 Phospho-RPA2 (Thr21)。Anti-Phospho-RPA2 (Thr21) Antibody (7P562) 可用于 ELISA, IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,300 | 5日内发货 | |
| 100 μL | ¥ 2,180 | 5日内发货 |
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抗原信息
| 产品描述 | Anti-Phospho-RPA2 (Thr21) Antibody (7P562) is an antibody targeting Phospho-RPA2 (Thr21). Anti-Phospho-RPA2 (Thr21) Antibody (7P562) can be used in ELISA, IF. |
| 别名 | Phospho-RPA2 (T21) |
| Ig Type | Rabbit IgG |
| 克隆号 | 7P562 |
| 交叉反应 | Human |
| 验证活性 | Immunofluorescence staining of Hela cells with TMAH-00958 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| 应用 | ELISA, IF |
| 推荐剂量 | IF:1:20-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Nucleus. Nucleus, PML body. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human Phospho-RPA2 (T21) |
| 抗原种属 | Human |
| 基因ID | 6118 |
| Uniprot ID | |
| 研究领域 | Epigenetics and Nuclear Signaling |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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