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Anti-Phospho-PTPN11 (Tyr542) Antibody (3Q687)
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产品编号 TMAH-00951
别名 PTPN11 (p-Y542), PTPN11 (p-Tyr542), p-PTPN11 (Y542), p-PTPN11 (Tyr542), Phospho-PTPN11 (Y542)
Anti-Phospho-PTPN11 (Tyr542) Antibody (3Q687) 是一种抗体，靶向 Phospho-PTPN11 (Tyr542)。Anti-Phospho-PTPN11 (Tyr542) Antibody (3Q687) 可用于 ELISA, WB, IP。
Anti-Phospho-PTPN11 (Tyr542) Antibody (3Q687)
Anti-Phospho-PTPN11 (Tyr542) Antibody (3Q687)
还可以产品编号 TMAH-00951 别名 PTPN11 (p-Y542), PTPN11 (p-Tyr542), p-PTPN11 (Y542), p-PTPN11 (Tyr542), Phospho-PTPN11 (Y542)
Anti-Phospho-PTPN11 (Tyr542) Antibody (3Q687) 是一种抗体，靶向 Phospho-PTPN11 (Tyr542)。Anti-Phospho-PTPN11 (Tyr542) Antibody (3Q687) 可用于 ELISA, WB, IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,305 | 5日内发货 | |
| 100 μL | ¥ 2,190 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Phospho-PTPN11 (Tyr542) Antibody (3Q687) is an antibody targeting Phospho-PTPN11 (Tyr542). Anti-Phospho-PTPN11 (Tyr542) Antibody (3Q687) can be used in ELISA, WB, IP. |
| 别名 | PTPN11 (p-Y542), PTPN11 (p-Tyr542), p-PTPN11 (Y542), p-PTPN11 (Tyr542), Phospho-PTPN11 (Y542) |
| Ig Type | Rabbit IgG |
| 克隆号 | 3Q687 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate(treated with Pervanadate or not) -All lanes Phospho-PTPN11 antibody at 0.65μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 68 KDa -Observed band size: 68 KDa 2. Immunoprecipitating Phospho-PTPN11 in Hela whole cell lysate treated with Pervanadate -Lane 1: Rabbit control IgG(1μg)instead of TMAH-00951 in Hela whole cell lysate treated with Pervanadate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) -Lane 2: TMAH-00951(3μg)+ Hela whole cell lysate treated with Pervanadate(1mg) -Lane 3: Hela whole cell lysate treated with Pervanadate(20μg) |
| 应用 | |
| 推荐剂量 | WB:1:500-1:5000; IP:1:200-1:1000. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Cytoplasm. Nucleus. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway. Dephosphorylates GAB1, ARHGAP35 and EGFR. Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity. Dephosphorylates CDC73. Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human Phospho-PTPN11 (Y542) |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Signal Transduction |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
SCI 文献
关键词
Tyrosine-protein phosphatase non-receptor type 11SH-PTP3SH-PTP2 (SHP-2;Shp2)SH-PTP2 (SHP2;Shp 2)SHPTP2SH-PTP 3SHPTP 2PTPN11 (pY542)PTPN11 (pTyr542)PTPN11 (phospho-Y542)PTPN11 (phospho-Tyr542)PTPN11PTPN 11PTP-2CPTP2CPTP-1DPTP1DProtein-tyrosine phosphatase 2C (PTP-2C)Protein-tyrosine phosphatase 1D (PTP-1D)pPTPN11 (Y542)pPTPN11 (Tyr542)
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