Anti-Phospho-Progesterone Receptor (Ser190) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-Progesterone Receptor (Ser190). Anti-Phospho-Progesterone Receptor (Ser190) Polyclonal Antibody 2 can be used in WB,FCM.

IgG

Human,Rat (predicted:Mouse,Cow)

1. Blank control: MCF7.
Primary Antibody (green line): Rabbit Anti-Phospho-Progesterone Receptor (Ser190) antibody (TMAB-11187)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. 25 μg total protein per lane of various lysates (see on figure) probed with Phospho-Progesterone Receptor (Ser190) polyclonal antibody, unconjugated (TMAB-11187) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: rat Progesterone Receptor around the phosphorylation site of Ser190

Rat

PGR

Progesterone receptor

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
Isoform A is inactive in stimulating c-Src/MAPK signaling on hormone stimulation.