Anti-Phospho-PRKN (Ser65) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PRKN (Ser65). Anti-Phospho-PRKN (Ser65) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Pig,Cow,Horse,Sheep,Monkey)

1. Sample: Thymus (Mouse) Lysate at 40 μg Liver (Mouse) Lysate at 40 μg Lung (Mouse) Lysate at 40 μg Skeletal muscle (Mouse) Lysate at 40 μg
Primary: Anti-phospho-Parkin (Ser65) (TMAB-01487) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
2. Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 μg
Lane 2: Lung (Mouse) Lysate at 40 μg
Lane 3: SH-SY5Y (Human) Cell Lysate at 30 μg
Lane 4: 293T (Human) Cell Lysate at 30 μg
Lane 5: MCF-7 (Human) Cell Lysate at 30 μg
Lane 6: Jurkat (Human) Cell Lysate at 30 μg
Primary: Anti-phospho-Parkin (Ser65) (TMAB-01487) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 50 kDa
3. Sample: A673 (Human) cell Lysate at 40 μg
Primary: Anti-p-Parkin (Ser65) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
4. Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Parkin (Ser65)) Polyclonal Antibody, Unconjugated (TMAB-01487) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthesised phosphopeptide: human Parkin around the phosphorylation site of Ser65

Human

Parkin / PARK,Mitophagy fission and fusion,RING Finger E3 Ligase