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Anti-Phospho-PRKCA (T638) Antibody (1C677) 是一种 Rabbit 抗体,靶向 Phospho-PRKCA (T638)。Anti-Phospho-PRKCA (T638) Antibody (1C677) 可用于 WB,ICC/IF,IHC,IP。
Anti-Phospho-PRKCA (T638) Antibody (1C677) 是一种 Rabbit 抗体,靶向 Phospho-PRKCA (T638)。Anti-Phospho-PRKCA (T638) Antibody (1C677) 可用于 WB,ICC/IF,IHC,IP。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,495 | 5日内发货 | |
100 μL | ¥ 2,480 | 5日内发货 |
产品描述 | Anti-Phospho-PRKCA (Thr638) Antibody (1C677) 是一种 Rabbit 抗体,靶向 Phospho-PRKCA (Thr638)。Anti-Phospho-PRKCA (Thr638) Antibody (1C677) 可用于 WB,ICC/IF,IHC,IP。 |
别名 | Protein kinase C zeta, Protein kinase C gamma, Protein kinase C epsilon, Protein kinase C delta, Protein kinase C beta, Protein kinase C alpha, Protein kinase C, PRKCZ, PRKCG, PRKCE, PRKCD, PRKCB2, PRKCB1, PRKCB, PRKCA (p-Thr638), PRKCA (p-T638), PRKCA, p-PRKCA (Thr638), p-PRKCA (T638), PKCG, PKCE, PKCD, PKCB, PKC-alpha, PKC-A, PKCA, PKC2, PKC zeta, PKC gamma, PKC epsilon, PKC delta, PKC beta, PKC alpha, Phospho-PRKCA (T638), KPCA_HUMAN |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of Phospho-PKC alpha (T638) on different lysates using anti-Phospho-PKC alpha (T638) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: 293. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-PKC alpha (T638) antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Phospho-PKC alpha (T638) antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-PKC alpha (T638) antibody. Counter stained with hematoxylin. 5. ICC staining Phospho-PKC alpha (T638) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Phospho-PKC alpha (T638) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Phospho-PKC alpha (T638) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
应用 | |
推荐剂量 | WB: 1:1000-5000; IHC: 1:50-500; ICC/IF: 1:50-500 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor-promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (α, βI, βII, γ, δ, ε, ζ, η, θ, λ/ι, μ and ν). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC δ and ε are independent of Ca2+. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | A synthesized phosphopeptide: human PKC alpha around the phosphorylation site of Thr638 |
抗原种属 | human |
Uniprot ID |
分子量 | Theoretical: 77 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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