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Anti-Phospho-PRKAR2A (S99) Antibody (9R534) 是一种 Rabbit 抗体,靶向 Phospho-PRKAR2A (S99)。Anti-Phospho-PRKAR2A (S99) Antibody (9R534) 可用于 WB,ICC/IF,IHC,IP。
Anti-Phospho-PRKAR2A (S99) Antibody (9R534) 是一种 Rabbit 抗体,靶向 Phospho-PRKAR2A (S99)。Anti-Phospho-PRKAR2A (S99) Antibody (9R534) 可用于 WB,ICC/IF,IHC,IP。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,490 | 5日内发货 | |
100 μL | ¥ 2,485 | 5日内发货 |
产品描述 | Anti-Phospho-PRKAR2A (Ser99) Antibody (9R534) 是一种 Rabbit 抗体,靶向 Phospho-PRKAR2A (Ser99)。Anti-Phospho-PRKAR2A (Ser99) Antibody (9R534) 可用于 WB,ICC/IF,IHC,IP。 |
别名 | PRKAR2A (p-Ser99), PRKAR2A (p-S99), p-PRKAR2A (Ser99), p-PRKAR2A (S99) |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat,Pig |
验证活性 | 1. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-phospho-PKA R2 (S99) antibody. Counter stained with hematoxylin. 2. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-phospho-PKA R2 (S99) antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-phospho-PKA R2 (S99) antibody. Counter stained with hematoxylin. 4. ICC staining phospho-PKA R2 (S99) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining phospho-PKA R2 (S99) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
应用 | |
推荐剂量 | WB: 1:500; IHC: 1:50-200; ICC/IF: 1:50-200 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Hybridoma Monoclonal Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | A synthesized phosphopeptide: human PKA R2 around the phosphorylation site of Ser99 |
抗原种属 | human |
Uniprot ID |
分子量 | Theoretical: 51 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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