Anti-Phospho-PLCγ1 (Tyr783) Antibody (8M617) is a Rabbit antibody targeting Phospho-PLCγ1 (Tyr783). Anti-Phospho-PLCγ1 (Tyr783) Antibody (8M617) can be used in WB.

Monoclonal Rabbit IgG

8M617

Human;

Species predicted to react based on 100% sequence homology: Mouse, Rat, Cynomolgus, Bovin

1. Western blot analysis of extracts from serum-starved A-431, untreated (-) or treated with EGF (50 ng/mL, 10min; +) , using Phospho-PLCγ1 (Tyr783) Antibody, Rabbit MAb at 1:1000 dilution (upper) or Beta-Tubulin Loading Control Antibody, Mouse MAb at 1:20000 dilution (lower).

2. Western blot analysis of extracts from serum-starved A-431, treated with EGF (50 ng/mL, 10min), using Phospho-PLCγ1 (Tyr783) Antibody, Rabbit MAb and other brands’ antibodies (Company C) at dilution of 1:1000, 1:10000.

3. Western blot analysis of extracts from serum-starved A431, untreated (line A); treated with EGF using Phospho-PLCγ1 (Tyr783) Antibody, Rabbit MAb at 1:1000 dilution. (Validation Experiment)

4. Western blot analysis of extracts from serum-starved A431, untreated (line A); treated with EGF or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-PLCγ1 (Tyr783) Antibody, Rabbit MAb at 1:1000 dilution. (Validation Experiment)

WB

WB: 1:500-1:5000

Monoclonal

Unconjugated

A synthetic phosphopeptide corresponding to residues around Tyr783 of the Human PLCγ1

Human