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Anti-Phospho-PAK1/2/3 (Ser139) Antibody (4F435)
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产品编号 TMAC-03246
别名 p-PAK1/PAK2/PAK3 (Ser139), p-PAK1/PAK2/PAK3 (S139), Phospho-PAK1/PAK2/PAK3 (S139), PAK1/PAK2/PAK3 (p-Ser139), PAK1/PAK2/PAK3 (p-S139)
Anti-Phospho-PAK1/2/3 (S139) Antibody (4F435) 是一种 Rabbit 抗体，靶向 Phospho-PAK1/2/3 (S139)。Anti-Phospho-PAK1/2/3 (S139) Antibody (4F435) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-Phospho-PAK1/2/3 (Ser139) Antibody (4F435)
Anti-Phospho-PAK1/2/3 (Ser139) Antibody (4F435)
还可以产品编号 TMAC-03246 别名 p-PAK1/PAK2/PAK3 (Ser139), p-PAK1/PAK2/PAK3 (S139), Phospho-PAK1/PAK2/PAK3 (S139), PAK1/PAK2/PAK3 (p-Ser139), PAK1/PAK2/PAK3 (p-S139)
Anti-Phospho-PAK1/2/3 (S139) Antibody (4F435) 是一种 Rabbit 抗体，靶向 Phospho-PAK1/2/3 (S139)。Anti-Phospho-PAK1/2/3 (S139) Antibody (4F435) 可用于 WB,ICC/IF,IHC,IP,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,495 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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偶联与修饰
抗原信息
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| 产品描述 | Anti-Phospho-PAK1/2/3 (Ser139) Antibody (4F435) 是一种 Rabbit 抗体，靶向 Phospho-PAK1/2/3 (Ser139)。Anti-Phospho-PAK1/2/3 (Ser139) Antibody (4F435) 可用于 WB,ICC/IF,IHC,IP,FCM。 |
| 别名 | p-PAK1/PAK2/PAK3 (Ser139), p-PAK1/PAK2/PAK3 (S139), Phospho-PAK1/PAK2/PAK3 (S139), PAK1/PAK2/PAK3 (p-Ser139), PAK1/PAK2/PAK3 (p-S139) |
| Ig Type | IgG |
| 克隆号 | 4F435 |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) on different lysates using anti-Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: NIH/3T3, Lane 3: SH-SY-5Y. 2. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Phospho-PAK1 (S144)+PAK2(S141)+PAK3 (S139) antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-PAK1(S144)+PAK2(S141)+PAK3(S139) antibody. Counter stained with hematoxylin. 5. ICC staining Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of NIH/3T3 cells with Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Cytoplasm, Cell membrane. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | Phospho |
| 免疫原 | A synthesized phosphopeptide: human PAK1 around the phosphorylation site of Ser144 |
| 抗原种属 | human |
| 分子量 | Theoretical: 65 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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