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Anti-Phospho-NFAT2 (Ser233) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-NFAT2 (Ser233)。Anti-Phospho-NFAT2 (Ser233) Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-Phospho-NFAT2 (Ser233) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-NFAT2 (Ser233)。Anti-Phospho-NFAT2 (Ser233) Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,165 | 5日内发货 | |
100 μL | ¥ 1,975 | 5日内发货 | |
200 μL | ¥ 2,780 | 5日内发货 |
产品描述 | Anti-Phospho-NFAT2 (Ser233) Polyclonal Antibody is a Rabbit antibody targeting Phospho-NFAT2 (Ser233). Anti-Phospho-NFAT2 (Ser233) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
交叉反应 | Human,Rat (predicted:Mouse,Pig,Cow,Sheep) |
验证活性 | 1. Sample: K562 (Human) Cell Lysate at 30 μg Primary: Anti-phospho-NFAT2 (Ser233) (Ser235 mouse)(TMAB-01461) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 101 kDa Observed band size: 101 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-NFAT2 (Ser233) (Ser235 mouse)) Polyclonal Antibody, Unconjugated (TMAB-01461) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. [provided by RefSeq, Jul 2013] |
免疫原 | KLH conjugated synthesised phosphopeptide: human NFAT2 around the phosphorylation site of Ser233 |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Transcription factors/regulators,NFATs,Other factors,Non-CD,NFATS |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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