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Anti-Phospho-MSK2 (Thr568) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-MSK2 (Thr568)。Anti-Phospho-MSK2 (Thr568) Polyclonal Antibody 可用于 WB,IHC。
Anti-Phospho-MSK2 (Thr568) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-MSK2 (Thr568)。Anti-Phospho-MSK2 (Thr568) Polyclonal Antibody 可用于 WB,IHC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,480 | 5日内发货 | |
100 μL | ¥ 2,240 | 5日内发货 |
产品描述 | Anti-Phospho-MSK2 (Thr568) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MSK2 (Thr568). Anti-Phospho-MSK2 (Thr568) Polyclonal Antibody can be used in WB,IHC. |
别名 | p-MSK2 (Thr568), p-MSK2 (T568), MSK2 (p-Thr568), MSK2 (p-T568) |
Ig Type | IgG |
交叉反应 | Human,Mouse |
验证活性 | 1. Western blot analysis of extracts from 293 cells, treated with H2O2 (100uM, 15mins), using MSK2 (Phospho-Thr568) antibody TMAC-02659. The lane on the right is treated with the synthesized peptide. 2. Immunohistochemistry analysis of paraffin-embedded human brain tissue using MSK2 (Phospho-Thr568) antibody TMAC-02659. The picture on the right is treated with the synthesized peptide. |
应用 | |
推荐剂量 | WB: 1:500-3000; IHC: 1:50-100 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
构建方式 | Polyclonal Antibody |
纯化方式 | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
性状 | Liquid |
缓冲液 | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | Peptide sequence around phosphorylation site of threonine 568 (M-Q-T(p)-P-C) derived from Human MSK2 |
抗原种属 | human |
Uniprot ID |
分子量 | Actual: 95 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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