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Anti-Phospho-MAP2K1 (S298) Antibody (9P567) 是一种 Rabbit 抗体,靶向 Phospho-MAP2K1 (S298)。Anti-Phospho-MAP2K1 (S298) Antibody (9P567) 可用于 WB,ICC/IF,IHC。
Anti-Phospho-MAP2K1 (S298) Antibody (9P567) 是一种 Rabbit 抗体,靶向 Phospho-MAP2K1 (S298)。Anti-Phospho-MAP2K1 (S298) Antibody (9P567) 可用于 WB,ICC/IF,IHC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,490 | 5日内发货 | |
100 μL | ¥ 2,485 | 5日内发货 |
产品描述 | Anti-Phospho-MAP2K1 (Ser298) Antibody (9P567) 是一种 Rabbit 抗体,靶向 Phospho-MAP2K1 (Ser298)。Anti-Phospho-MAP2K1 (Ser298) Antibody (9P567) 可用于 WB,ICC/IF,IHC。 |
别名 | p-MAP2K1 (Ser298), p-MAP2K1 (S298), MAP2K1 (p-Ser298), MAP2K1 (p-S298) |
Ig Type | IgG |
克隆号 | 9P567 |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of Phospho-MEK1 (S298) on different lysates using anti-Phospho-MEK1 (S298) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: NIH/3T3, Lane 3: A431. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-MEK1 (S298) antibody. Counter stained with hematoxylin. 3. ICC staining Phospho-MEK1 (S298) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining Phospho-MEK1 (S298) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Phospho-MEK1 (S298) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
应用 | |
推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | A synthesized phosphopeptide: human MEK1 around the phosphorylation site of Ser298 |
抗原种属 | human |
Uniprot ID |
分子量 | Theoretical: 43 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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