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Anti-Phospho-Histone H1.3/1.4 (T17) Antibody (6O691) 是一种 Rabbit 抗体,靶向 Phospho-Histone H1.3/1.4 (T17)。Anti-Phospho-Histone H1.3/1.4 (T17) Antibody (6O691) 可用于 WB,ICC/IF,IHC。
Anti-Phospho-Histone H1.3/1.4 (T17) Antibody (6O691) 是一种 Rabbit 抗体,靶向 Phospho-Histone H1.3/1.4 (T17)。Anti-Phospho-Histone H1.3/1.4 (T17) Antibody (6O691) 可用于 WB,ICC/IF,IHC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,480 | 5日内发货 | |
100 μL | ¥ 2,490 | 5日内发货 |
产品描述 | Anti-Phospho-Histone H1.3/1.4 (Thr17) Antibody (6O691) 是一种 Rabbit 抗体,靶向 Phospho-Histone H1.3/1.4 (Thr17)。Anti-Phospho-Histone H1.3/1.4 (Thr17) Antibody (6O691) 可用于 WB,ICC/IF,IHC。 |
别名 | Phospho-Histone H1.4 (T17), p-Histone H1.4 (Thr17), p-Histone H1.4 (T17), MGC116819, Histone H1B, Histone H1.4 (p-Thr17), Histone H1.4 (p-T17), Histone H1, Histone cluster 1 H1e, Histone 1 H1e, Hist1h1e, H1F4, H1E, H14_HUMAN, H1.4, H1 histone family member 4 |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) on CRC cell lysates using anti-Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) antibody at 1/500 dilution. Positive control: Lane 1: Untreated CRC whole cell lysates, Lane2: CRC cells treated with 1.5ug/ml Colcemid for 12 hours whole cell lysates. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse colon cancer tissue using anti-Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) antibody. Counter stained with hematoxylin. 4. ICC staining Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) in CRC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
应用 | |
推荐剂量 | WB: 1:500-1000; IHC: 1:50-200; ICC/IF: 1:50-200 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | A synthesized phosphopeptide: human H1.4 around the phosphorylation site of Thr17 |
抗原种属 | human |
分子量 | Theoretical: 30 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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