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Anti-Phospho-EIF4E (Ser209) Antibody (4B622)

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Anti-Phospho-EIF4E (Ser209) Antibody (4B622)
产品编号 TMAC-03211
别名 Phospho-EIF4E (S209), p-EIF4E (Ser209), p-EIF4E (S209), mRNA cap binding protein, MGC111573, Messanger RNA Cap Binding Protein eIF 4E, IF4E_HUMAN, Eukaryotic translation initiation factor 4E like 1, Eukaryotic translation initiation factor 4E, Eukaryotic translation initiation factor 4 E, EIF4F, EIF4EL1, EIF4E1, EIF4E (p-Ser209), EIF4E (p-S209), eIF 4F 25 kDa subunit, EIF 4F, CBP, AUTS19

Anti-Phospho-EIF4E (S209) Antibody (4B622) 是一种 Rabbit 抗体,靶向 Phospho-EIF4E (S209)。Anti-Phospho-EIF4E (S209) Antibody (4B622) 可用于 WB,ICC,IHC,IP。

Anti-Phospho-EIF4E (Ser209) Antibody (4B622)

Anti-Phospho-EIF4E (Ser209) Antibody (4B622)

Rating icon 还可以
Anti-Phospho-EIF4E (Ser209) Antibody (4B622)
产品编号 TMAC-03211 别名 Phospho-EIF4E (S209), p-EIF4E (Ser209), p-EIF4E (S209), mRNA cap binding protein, MGC111573, Messanger RNA Cap Binding Protein eIF 4E, IF4E_HUMAN, Eukaryotic translation initiation factor 4E like 1, Eukaryotic translation initiation factor 4E, Eukaryotic translation initiation factor 4 E, EIF4F, EIF4EL1, EIF4E1, EIF4E (p-Ser209), EIF4E (p-S209), eIF 4F 25 kDa subunit, EIF 4F, CBP, AUTS19

Anti-Phospho-EIF4E (S209) Antibody (4B622) 是一种 Rabbit 抗体,靶向 Phospho-EIF4E (S209)。Anti-Phospho-EIF4E (S209) Antibody (4B622) 可用于 WB,ICC,IHC,IP。

规格价格库存数量
50 μL
¥ 1,495
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100 μL
¥ 2,490
5日内发货
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产品介绍

生物活性
产品描述
Anti-Phospho-EIF4E (Ser209) Antibody (4B622) 是一种 Rabbit 抗体,靶向 Phospho-EIF4E (Ser209)。Anti-Phospho-EIF4E (Ser209) Antibody (4B622) 可用于 WB,ICC,IHC,IP。
别名Phospho-EIF4E (S209), p-EIF4E (Ser209), p-EIF4E (S209), mRNA cap binding protein, MGC111573, Messanger RNA Cap Binding Protein eIF 4E, IF4E_HUMAN, Eukaryotic translation initiation factor 4E like 1, Eukaryotic translation initiation factor 4E, Eukaryotic translation initiation factor 4 E, EIF4F, EIF4EL1, EIF4E1, EIF4E (p-Ser209), EIF4E (p-S209), eIF 4F 25 kDa subunit, EIF 4F, CBP, AUTS19
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of Phospho-eIF4E (S209) on different lysates using anti-Phospho-eIF4E (S209) antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse spleen, Lane 2: Rat spleen.
2. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Phospho-eIF4E (S209) antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-Phospho-eIF4E (S209) antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Phospho-eIF4E (S209) antibody. Counter stained with hematoxylin.
5. ICC staining Phospho-eIF4E (S209) in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining Phospho-eIF4E (S209) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining Phospho-eIF4E (S209) in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
应用
推荐剂量
WB: 1:1000-5000; IHC: 1:50-200; ICC: 1:50-200
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4E, eIF4A and eIF4G. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E binds to the mRNA "cap" during an early step in the initiation of protein synthesis. eIF4A acts as an ATP-dependent RNA helicase. eIF4G acts as a bridge between eIF4E, eIF4A and the eIF3 complex.
偶联与修饰
偶联
Unconjugated
其他可选修饰符
Phospho
抗原信息
免疫原
A synthesized phosphopeptide: human eIF4E around the phosphorylation site of Ser209
抗原种属
human
Uniprot ID
化学信息
分子量Theoretical: 25 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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