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Anti-Phospho-EIF4E (S209) Antibody (4B622) 是一种 Rabbit 抗体,靶向 Phospho-EIF4E (S209)。Anti-Phospho-EIF4E (S209) Antibody (4B622) 可用于 WB,ICC,IHC,IP。
Anti-Phospho-EIF4E (S209) Antibody (4B622) 是一种 Rabbit 抗体,靶向 Phospho-EIF4E (S209)。Anti-Phospho-EIF4E (S209) Antibody (4B622) 可用于 WB,ICC,IHC,IP。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,495 | 5日内发货 | |
100 μL | ¥ 2,490 | 5日内发货 |
产品描述 | Anti-Phospho-EIF4E (Ser209) Antibody (4B622) 是一种 Rabbit 抗体,靶向 Phospho-EIF4E (Ser209)。Anti-Phospho-EIF4E (Ser209) Antibody (4B622) 可用于 WB,ICC,IHC,IP。 |
别名 | Phospho-EIF4E (S209), p-EIF4E (Ser209), p-EIF4E (S209), mRNA cap binding protein, MGC111573, Messanger RNA Cap Binding Protein eIF 4E, IF4E_HUMAN, Eukaryotic translation initiation factor 4E like 1, Eukaryotic translation initiation factor 4E, Eukaryotic translation initiation factor 4 E, EIF4F, EIF4EL1, EIF4E1, EIF4E (p-Ser209), EIF4E (p-S209), eIF 4F 25 kDa subunit, EIF 4F, CBP, AUTS19 |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of Phospho-eIF4E (S209) on different lysates using anti-Phospho-eIF4E (S209) antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse spleen, Lane 2: Rat spleen. 2. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Phospho-eIF4E (S209) antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-Phospho-eIF4E (S209) antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Phospho-eIF4E (S209) antibody. Counter stained with hematoxylin. 5. ICC staining Phospho-eIF4E (S209) in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Phospho-eIF4E (S209) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Phospho-eIF4E (S209) in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
应用 | |
推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC: 1:50-200 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4E, eIF4A and eIF4G. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E binds to the mRNA "cap" during an early step in the initiation of protein synthesis. eIF4A acts as an ATP-dependent RNA helicase. eIF4G acts as a bridge between eIF4E, eIF4A and the eIF3 complex. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | A synthesized phosphopeptide: human eIF4E around the phosphorylation site of Ser209 |
抗原种属 | human |
Uniprot ID |
分子量 | Theoretical: 25 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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