Anti-Phospho-EIF2S1 (Ser51) Polyclonal Antibody is a Rabbit antibody targeting Phospho-EIF2S1 (Ser51). Anti-Phospho-EIF2S1 (Ser51) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Cow)

1. Sample: Testis (Mouse) Lysate at 40 μg
Primary: Anti-Phospho-eIF2 alpha (Ser51) (TMAB-01404) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 34 kDa
2. Sample: Ovary (Mouse) Lysate at 40 μg
Primary: Anti-Phospho-eIF2 alpha (Ser51) (TMAB-01404) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 34 kDa
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (Phospho-eIF2 alpha (Ser51)) Polyclonal Antibody, Unconjugated (TMAB-01404) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (Phospho-eIF2 alpha (Ser51)) Polyclonal Antibody, Unconjugated (TMAB-01404) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthesised phosphopeptide: human eIF2 alpha around the phosphorylation site of Ser51

Human

RNAi,Regulation,Eukaryotic Initiation factors (eIF's)