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Anti-Phospho-EIF2S1 (Ser51) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-EIF2S1 (Ser51)。Anti-Phospho-EIF2S1 (Ser51) Polyclonal Antibody 可用于 IF,WB,IHC,ELISA。
Anti-Phospho-EIF2S1 (Ser51) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-EIF2S1 (Ser51)。Anti-Phospho-EIF2S1 (Ser51) Polyclonal Antibody 可用于 IF,WB,IHC,ELISA。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,480 | 5日内发货 | |
100 μL | ¥ 2,230 | 5日内发货 |
产品描述 | Anti-Phospho-EIF2S1 (Ser51) Polyclonal Antibody is a Rabbit antibody targeting Phospho-EIF2S1 (Ser51). Anti-Phospho-EIF2S1 (Ser51) Polyclonal Antibody can be used in IF,WB,IHC,ELISA. |
别名 | Phospho-EIF2S1 (S51), p-EIF2S1 (Ser51), p-EIF2S1 (S51), IF2A_HUMAN, Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa, EIF2S1 (p-Ser51), EIF2S1 (p-S51), EIF2A, EIF2 alpha, EIF2, EIF 2alpha, EIF 2A, EIF 2 alpha, EIF 2 |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat,Dog,Pig,FiSh |
验证活性 | 1. Western blot analysis of extracts from Jurkat cells untreated or treated with H2O2 using eIF2a (Phospho-Ser51) Antibody TMAC-01265. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2α (Phospho-Ser51) Antibody TMAC-01265 (left) or the same antibody preincubated with blocking peptide (right). 3. Immunofluorescence staining of methanol-fixed Hela cells using eIF2a (Phospho-Ser51) Antibody TMAC-01265. 4. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using eIF2α (Phospho-Ser51) Antibody TMAC-01265. |
应用 | |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
构建方式 | Polyclonal Antibody |
纯化方式 | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
性状 | Liquid |
缓冲液 | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | Peptide sequence around phosphorylation site of serine 51 (E-L-S(p)-R-R) derived from Human eIF2a |
抗原种属 | human |
Uniprot ID |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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