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顶部生物活性储存和溶解信息计算器

Anti-Phospho-CHEK1 (Ser286) Polyclonal Antibody

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产品编号 TMAC-00834

别名 p-CHEK1 (Ser286), p-CHEK1 (S286), CHEK1 (p-Ser286), CHEK1 (p-S286)

Anti-Phospho-CHEK1 (Ser286) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-CHEK1 (Ser286)。Anti-Phospho-CHEK1 (Ser286) Polyclonal Antibody 可用于 ELISA,IF,WB。

Anti-Phospho-CHEK1 (Ser286) Polyclonal Antibody

Anti-Phospho-CHEK1 (Ser286) Polyclonal Antibody

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Rating icon 还可以
产品编号 TMAC-00834 别名 p-CHEK1 (Ser286), p-CHEK1 (S286), CHEK1 (p-Ser286), CHEK1 (p-S286)

Anti-Phospho-CHEK1 (Ser286) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-CHEK1 (Ser286)。Anti-Phospho-CHEK1 (Ser286) Polyclonal Antibody 可用于 ELISA,IF,WB。

规格价格库存数量
50 μL
¥ 1,490
5日内发货
100 μL
¥ 2,245
5日内发货
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产品介绍

生物活性
产品描述
Anti-Phospho-CHEK1 (Ser286) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CHEK1 (Ser286). Anti-Phospho-CHEK1 (Ser286) Polyclonal Antibody can be used in ELISA,IF,WB.
别名p-CHEK1 (Ser286), p-CHEK1 (S286), CHEK1 (p-Ser286), CHEK1 (p-S286)
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and NonPhosphopeptide (Phospho-right), using Chk1 (Phospho-Ser286) Antibody.
2. Immunofluorescence analysis of HeLa cells, using Chk1 (Phospho-Ser286) Antibody. The picture on the right is blocked with the phospho peptide.
3. Western blot analysis of lysates from K562 cells treated with Na3VO4 0.3uM 40', using Chk1 (Phospho-Ser286) Antibody. The lane on the right is blocked with the phospho peptide.
verifiedActivityverifiedActivityverifiedActivity
应用ELISAIFWB
推荐剂量
WB: 1:500-2000; IF: 1:200-1000; ELISA: 1:5000
抗体种类
Polyclonal
宿主来源Rabbit
构建方式Polyclonal Antibody
纯化方式The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
性状Liquid
缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
研究背景Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.
偶联与修饰
偶联
Unconjugated
其他可选修饰符
Phospho
抗原信息
免疫原
A synthesized phosphopeptide: human Chk1 around the phosphorylation site of Ser286. AA range:256-305
抗原种属
human
Uniprot ID
O14757
化学信息
分子量Actual: 55 kDa.
存储&运输
储存方式Store at -15°C to -25°C for 12 months (Do not lower than -25°C). Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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关键词

pCHEK1 (Ser286)pCHEK1 (S286)CHEK1 (pSer286)CHEK1 (pS286)CHEK1 (phospho-Ser286)CHEK1 (phospho-S286)

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